Nanobioelectronics & Biosensors Group, Institut Català de Nanociència I Nanotecnologia (ICN2), CSIC and The Barcelona Institute of Science and Technology (BIST), Campus UAB, Bellaterra, 08193 Barcelona, Spain.
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, Spain.
Chem Rev. 2022 Sep 28;122(18):14881-14910. doi: 10.1021/acs.chemrev.1c01012. Epub 2022 Sep 6.
Lateral flow assays (LFAs) are currently the most used point-of-care sensors for both diagnostic (e.g., pregnancy test, COVID-19 monitoring) and environmental (e.g., pesticides and bacterial monitoring) applications. Although the core of LFA technology was developed several decades ago, in recent years the integration of novel nanomaterials as signal transducers or receptor immobilization platforms has brought improved analytical capabilities. In this Review, we present how nanomaterial-based LFAs can address the inherent challenges of point-of-care (PoC) diagnostics such as sensitivity enhancement, lowering of detection limits, multiplexing, and quantification of analytes in complex samples. Specifically, we highlight the strategies that can synergistically solve the limitations of current LFAs and that have proven commercial feasibility. Finally, we discuss the barriers toward commercialization and the next generation of LFAs.
侧向流检测（LFA）是目前最常用于即时检测（例如妊娠检测、COVID-19 监测）和环境（例如农药和细菌监测）应用的床边传感器。尽管 LFA 技术的核心几十年前就已开发出来，但近年来新型纳米材料作为信号转导器或受体固定化平台的集成，带来了分析能力的提升。在这篇综述中，我们展示了基于纳米材料的侧向流检测如何解决即时检测诊断固有的挑战，例如灵敏度提高、检测限降低、多指标检测以及复杂样本中分析物的定量检测。具体来说，我们强调了能够协同解决当前侧向流检测局限性且已被证明具有商业可行性的策略。最后，我们讨论了迈向商业化和下一代侧向流检测的障碍。
