Wang Ying, Geng Zhen, Huang Yongcan, Jia Zhaojun, Cui Zhenduo, Li Zhaoyang, Wu Shuilin, Liang Yanqin, Zhu Shengli, Yang Xianjin, Lu William Weijia
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300350, China.
J Mater Chem B. 2018 Nov 7;6(41):6615-6621. doi: 10.1039/c8tb01746h. Epub 2018 Oct 2.
Magnesium (Mg) alloys, having a unique combination of strength and degradation, are being explored for various craniofacial and orthopedic applications. Nevertheless, the underlying mechanism of Mg to stimulate bone formation needs further investigation. In this in vitro study, the degradation behavior of pure Mg and the effect of Mg on the activity of osteoblasts were elucidated. From the corrosion test, it was determined that the degradation of pure Mg was able to create an alkaline microenvironment. It was further determined that Mg promoted the proliferation and differentiation of osteoblasts. By western blotting analysis, it was noted that Mg increased the phosphorylation of ERK (enhanced the c-fos level) and induced GSK3β phosphorylation (enhanced the β-catenin levels). These results demonstrated that the degradation of Mg was able to promote the proliferation and differentiation of osteoblasts, which may be related to the newly created alkaline microenvironment and the osteogenesis potential of released Mg through the MAPK/ERK signaling pathway.
镁（Mg）合金具有强度与降解特性的独特组合，正被探索用于各种颅面和骨科应用。然而，镁刺激骨形成的潜在机制仍需进一步研究。在这项体外研究中，阐明了纯镁的降解行为以及镁对成骨细胞活性的影响。通过腐蚀试验确定，纯镁的降解能够产生碱性微环境。进一步确定镁促进了成骨细胞的增殖和分化。通过蛋白质印迹分析发现，镁增加了ERK的磷酸化（提高了c-fos水平）并诱导了GSK3β磷酸化（提高了β-连环蛋白水平）。这些结果表明，镁的降解能够促进成骨细胞的增殖和分化，这可能与新产生的碱性微环境以及通过MAPK/ERK信号通路释放的镁的成骨潜力有关。
