Li Xin, Shen Fangyu, Wang Kebing, Lin Shuang, Zhou Lei, Chen Si, Wang Jin, Huang Nan
Key Laboratories of Advanced Technology for Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China.
J Mater Chem B. 2018 Dec 14;6(46):7582-7593. doi: 10.1039/c8tb01976b. Epub 2018 Nov 7.
Nitric oxide (NO), which is continuously released from the normal healthy endodermis cell layer of the vascular system, plays a crucial role in the stability and health maintenance of blood vessels. It is one of the most important gaseous signaling molecules and regulates cardiovascular homeostasis, inhibits blood clotting and intimal hyperplasia, and prevents atherosclerosis. Insufficient NO production is often observed in atherosclerosis lesions. In this work, a NO-generating bioactive coating built by a polydopamine film (PDA) and a copper ion was fabricated. The coating (Cu/PDA) had glutathione peroxidase (GPx)-like activity and was able to catalyze NO release from S-nitrosothiols (RSNOs) due to the catalytic activity of the copper ion. It was also capable of catalyzing RSNO decomposition and NO production. The copper ion was embedded in the PDA coating to ensure the effectiveness of long-term NO-catalytic activity. The surface exhibited a favorable suppression of vascular smooth muscle cell (VSMC) proliferation and also efficiently reduced thrombosis formation. Additionally, the NO catalytic surface had a positive influence on endothelial cell (EC) growth behavior. The in vivo study verified that the modified surface promoted healthy endothelium formation and suppressed intimal hyperplasia, which is conducive to re-endothelialization and for reducing restenosis of vascular stents. This work provides a potential strategy for the development of novel cardiovascular implants.
一氧化氮（NO）由血管系统正常健康的内皮细胞层持续释放，在血管的稳定性和健康维持中起着关键作用。它是最重要的气体信号分子之一，调节心血管稳态，抑制血液凝固和内膜增生，并预防动脉粥样硬化。在动脉粥样硬化病变中常观察到一氧化氮产生不足。在这项工作中，制备了一种由聚多巴胺膜（PDA）和铜离子构建的产生NO的生物活性涂层。该涂层（Cu/PDA）具有谷胱甘肽过氧化物酶（GPx）样活性，由于铜离子的催化活性，能够催化从亚硝基硫醇（RSNOs）释放NO。它还能够催化RSNO分解并产生NO。铜离子嵌入PDA涂层中以确保长期NO催化活性的有效性。该表面对血管平滑肌细胞（VSMC）增殖表现出良好的抑制作用，并且还能有效减少血栓形成。此外，NO催化表面对内皮细胞（EC）生长行为有积极影响。体内研究证实，修饰后的表面促进健康内皮形成并抑制内膜增生，这有利于再内皮化并减少血管支架的再狭窄。这项工作为新型心血管植入物的开发提供了一种潜在策略。
