Fan Dawei, Bao Chunzhu, Liu Xin, Wu Dan, Zhang Yong, Wang Huan, Du Bin, Wei Qin
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
J Mater Chem B. 2018 Dec 14;6(46):7634-7642. doi: 10.1039/c8tb02122h. Epub 2018 Nov 5.
A unique hierarchical mesoporous SnO microflower was utilized for the fabrication of an immunosensor. The modified nitrogen-doped carbon quantum dots (NCQDs) improved the photoelectrochemical (PEC) activity of the SnO, and contributed to the in situ assembly of BiS nanoparticles on the surface of the SnO materials. Under the stimulation of visible light and ascorbic acid as an efficient electron donor, the NCQDs and BiS co-sensitized mesoporous SnO microflower (SnO/NCQDs/BiS) modified ITO electrode exhibited an excellent photoelectric signal arriving at around 150 μA. Moreover, a novel label-free PEC sensor based SnO/NCQDs/BiS composite was constructed, which enabled the ultrasensitive detection of N-terminal pro-B-type natriuretic peptide (NT-proBNP). The photocurrents decreased linearly with the logarithm of NT-proBNP concentration in the range of 0.01-50 ng mL with a detection limit of 3.7 pg mL (S/N = 3). Furthermore, the novel PEC immunosensor showed perfect selectivity, good stability and reproducibility, which suggest promising applications in the sensing and clinical diagnosis of cardiac disease.
一种独特的分级介孔SnO微花被用于制备免疫传感器。修饰后的氮掺杂碳量子点（NCQDs）提高了SnO的光电化学（PEC）活性，并有助于BiS纳米颗粒在SnO材料表面原位组装。在可见光和作为有效电子供体的抗坏血酸的刺激下，NCQDs和BiS共敏化的介孔SnO微花（SnO/NCQDs/BiS）修饰的ITO电极表现出优异的光电信号，达到约150 μA。此外，构建了一种基于SnO/NCQDs/BiS复合材料的新型无标记PEC传感器，能够超灵敏检测N端前B型利钠肽（NT-proBNP）。在0.01-50 ng/mL范围内，光电流随NT-proBNP浓度的对数呈线性下降，检测限为3.7 pg/mL（S/N = 3）。此外，新型PEC免疫传感器表现出完美的选择性、良好的稳定性和重现性，这表明其在心脏病传感和临床诊断中具有广阔的应用前景。
