Li Hao, Zhang Mengling, Song Yuxiang, Wang Huibo, Liu Chang'an, Fu Yijun, Huang Hui, Liu Yang, Kang Zhenhui
Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Institute of Functional Nano and Soft Materials (FUNSOM), Soochow University, Suzhou 215123, China.
J Mater Chem B. 2018 Sep 28;6(36):5708-5717. doi: 10.1039/c8tb01751d. Epub 2018 Aug 24.
Multifunctional carbon dots (CDs) with lifetime thermal sensing, nucleolus imaging, and antialgal activity properties were synthesized directly from ascorbic acid aqueous solution by a one-step electrochemical method at room temperature. The as-prepared CDs are responsive to temperature and exhibit an accurate linear response of fluorescence intensity vs. temperature (20-100 °C). These CDs can enter a cell and nucleolus, adsorb on the nucleic acids (DNA and RNA) and the fluorescence intensity of CDs is increased by the adsorption of nucleic acids. In addition, the CDs can inhibit the activity of RuBisCO in Anabaena sp., leading to reducing the growth of Anabaena sp. All these properties make the CDs serve as effective fluorescence-based nanothermometers, nucleolus probes, and antialgal agents.
通过一步电化学法在室温下由抗坏血酸水溶液直接合成了具有寿命热传感、核仁成像和抗藻活性特性的多功能碳点。所制备的碳点对温度有响应，并在20-100°C范围内呈现荧光强度与温度的精确线性响应。这些碳点可以进入细胞和核仁，吸附在核酸（DNA和RNA）上，并且核酸的吸附会增加碳点的荧光强度。此外，碳点可以抑制鱼腥藻中核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO）的活性，从而导致鱼腥藻生长减缓。所有这些特性使碳点能够作为基于荧光的有效纳米温度计、核仁探针和抗藻剂。
