Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, P. R. China.
J Mater Chem B. 2019 Feb 14;7(6):940-952. doi: 10.1039/c8tb02938e. Epub 2019 Jan 15.
Many pathophysiologic conditions can interrupt the normal wound healing process and lead to chronic wounds due to the arrest of macrophages in their inflammatory phenotype. Thus, strategies that promote the recovery of macrophage functions are of great benefit to heal chronic wounds. Bioactive glass (BG) dissolution has been recognized for its modulatory functions in macrophage polarization. However, further efforts are greatly needed to address how BG particles affect macrophage behaviors (such as proliferation, viability, migration and polarization) and wound healing through both direct contact and released ions. Our results showed that BG particles affect the proliferation/viability and polarization of macrophages in a dose-dependent manner. At a low concentration (20 μg mL), BG particles stimulated macrophage proliferation and promoted the M1-to-M2 phenotype switch; meanwhile, at a high concentration (100 μg mL), the particles showed significant cytotoxicity and prolonged the M1 phenotype of macrophages. The BG particles also exhibited strong chemotaxis to macrophages which appeared to be independent of their concentration. Dose-dependent regulation of macrophages and wound closure by BG particles were also observed in the healing of full-thickness wounds of diabetic rats. Our study suggests that the BG particle-mediated activities of macrophages are essential to wound healing, and these activities are greatly correlated with the amount of BG particles.
许多病理生理状况可通过巨噬细胞在炎症表型中的停滞来中断正常的伤口愈合过程，导致慢性伤口。因此，促进巨噬细胞功能恢复的策略对于慢性伤口的愈合非常有益。生物活性玻璃（BG）的溶解已被认为具有调节巨噬细胞极化的功能。然而，仍需要进一步努力来解决 BG 颗粒如何通过直接接触和释放离子影响巨噬细胞行为（如增殖、活力、迁移和极化）和伤口愈合。我们的结果表明，BG 颗粒以剂量依赖的方式影响巨噬细胞的增殖/活力和极化。在低浓度（20μg/mL）下，BG 颗粒刺激巨噬细胞增殖并促进 M1 向 M2 表型转换；同时，在高浓度（100μg/mL）下，颗粒表现出明显的细胞毒性并延长巨噬细胞的 M1 表型。BG 颗粒对巨噬细胞也表现出强烈的趋化作用，这种作用似乎与其浓度无关。在糖尿病大鼠全层伤口愈合中也观察到 BG 颗粒对巨噬细胞的剂量依赖性调节和伤口闭合。我们的研究表明，BG 颗粒介导的巨噬细胞活性对伤口愈合至关重要，这些活性与 BG 颗粒的数量密切相关。
