Department of Oral and Cranio-Maxillofacial Science, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China.
J Mater Chem B. 2019 Apr 21;7(15):2518-2533. doi: 10.1039/c8tb02850h. Epub 2019 Mar 19.
Bone implants play a crucial role in bone repairing. Nevertheless, low capability of osteoinductivity and osteogenic differentiation for bone regeneration are disadvantages of bone implants. Therefore, it is imperative to develop a general and facile technology to promote the bioactivity of existing implants. Herein, a facile amorphous carbon-coating approach was developed to stimulate osteogenesis on diverse biomaterials, including bioceramics, biometals, and biopolymers via magnetron sputtering deposition. The results confirmed that the amorphous carbon-coating-modified surfaces could significantly enhance osteogenesis of bone marrow mesenchymal stem cells (BMSCs) on every kind of biomaterial surface. Furthermore, it was demonstrated that the FAK/ERK1/2 signaling pathways were involved in the osteogenic effects of this amorphous carbon coating. The bone regeneration ability using the calvarial bone defect model of rats confirmed that the amorphous carbon coating induced faster bone formation and mineralization, which suggested the effect of amorphous carbon coating on stimulating osteogenesis in vivo. These results suggest that the approach involving modifying a surface with amorphous carbon provides a general and simple strategy to enhance the osteogenesis for diverse biomaterials, and this has promising potential for bone repairing applications.
骨植入物在骨修复中起着至关重要的作用。然而，对于骨再生来说，其成骨诱导能力和骨生成分化能力低是其缺点。因此，开发一种通用且简便的技术来提高现有植入物的生物活性是当务之急。在此，通过磁控溅射沉积开发了一种简便的非晶碳涂层方法，以刺激包括生物陶瓷、生物金属和生物聚合物在内的各种生物材料的成骨作用。结果证实，非晶碳涂层改性表面可以显著增强骨髓间充质干细胞（BMSCs）在各种生物材料表面的成骨作用。此外，研究表明，FAK/ERK1/2 信号通路参与了这种非晶碳涂层的成骨作用。使用大鼠颅骨骨缺损模型证实了骨再生能力，表明非晶碳涂层诱导更快的骨形成和矿化，这表明非晶碳涂层在体内刺激成骨的作用。这些结果表明，用非晶碳修饰表面的方法为提高各种生物材料的成骨作用提供了一种通用且简便的策略，这对于骨修复应用具有广阔的应用前景。
