Zou Mengqi, Li Daxiu, Yuan Ruo, Xiang Yun
Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
J Mater Chem B. 2018 Jun 28;6(24):4146-4150. doi: 10.1039/c8tb00610e. Epub 2018 Jun 8.
Highly sensitive and selective detection of protein disease markers is crucial for fundamental research and disease diagnosis. In this work, based on a new target-triggered autonomous aptamer machinery amplification approach, we developed a simple and sensitive sensing platform for the detection of a cancer biomarker, platelet-derived growth factor BB (PDGF-BB), in human sera. The target PDGF-BB binds the fluorescently quenched hairpin aptamer probes and causes structural and conformational changes in the aptamers to recover their fluorescence. The presence of partial duplex DNA fuel strands further leads to the recycling of the target proteins and the functioning of the aptamer machine autonomously to amplify the fluorescence emission significantly, thereby resulting in sensitive detection of PDGF-BB down to the low picomolar level. Besides, due to the high specificity of the aptamer, such a protein detection method shows high selectivity and can be employed to detect PDGF-BB in human sera. Featuring the advantages of being enzyme-free and nanomaterial-free for signal amplification, as well as homogeneous detection, the developed approach thus holds great potential for the construction of various aptasensors for the convenient and sensitive detection of different molecules.
蛋白质疾病标志物的高灵敏和高选择性检测对于基础研究和疾病诊断至关重要。在这项工作中，基于一种新的靶标触发的自主适配体机器扩增方法，我们开发了一种简单且灵敏的传感平台，用于检测人血清中的一种癌症生物标志物——血小板衍生生长因子BB（PDGF-BB）。靶标PDGF-BB与荧光淬灭的发夹适配体探针结合，导致适配体的结构和构象发生变化，从而恢复其荧光。部分双链DNA燃料链的存在进一步导致靶标蛋白的循环利用以及适配体机器的自主运行，从而显著放大荧光发射，进而实现对低至皮摩尔水平的PDGF-BB的灵敏检测。此外，由于适配体的高特异性，这种蛋白质检测方法具有高选择性，可用于检测人血清中的PDGF-BB。所开发的方法具有无需酶和纳米材料进行信号放大以及均相检测的优点，因此在构建各种用于便捷、灵敏检测不同分子的适配体传感器方面具有巨大潜力。
