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绿豆黄花叶印度病毒感染后不同品种的差异反应。

Differential responses of cultivars following mungbean yellow mosaic India virus infection.

作者信息

Patwa Nisha, Chatterjee Chitra, Basak Jolly

机构信息

1Department of Biotechnology, Visva-Bharati, Siksha Bhavana, Santiniketan, West Bengal 731235 India.

2Present Address: Horticultural Insects Research Laboratory, USDA-ARS, Application Technology Research Unit, 1680 Madison Ave., Wooster, OH 44691 USA.

出版信息

Physiol Mol Biol Plants. 2020 Apr;26(4):817-828. doi: 10.1007/s12298-019-00741-w. Epub 2020 Jan 1.

Abstract

commonly known as French bean is a vital leguminous crop worldwide and India stood 1st rank in dry bean and 4th rank in green bean production worldwide (FAOSTAT 2017). However, this production is severely affected by Mungbean yellow mosaic India virus (MYMIV) infection. Hence it is very important to identify MYMIV tolerant cultivars. MYMIV infection results in the production of reactive oxygen species and plant cells have evolved complex defense mechanisms at different levels to overcome the damage. Our study for the first time focused on the changes at the morphological and biochemical level, as well as on the relative quantification of MYMIV genes in nine cultivars of after MYMIV infection. Highest growth and the highest accumulation of four antioxidants of cv. 'Anupam' after MYMIV infection, established that cv. 'Anupam' was less affected by MYMIV infection amongst all nine cultivars. Relative quantification studies also correlated well with these results. Additionally, there is a consistent level of photosynthetic pigments content in mock- and MYMIV-treated seedlings of cv. 'Anupam' over early infection period. Combining all the results we conclude that cv. 'Anupam' is a MYMIV tolerant cultivar.

摘要

通常被称为法国豆,是全球重要的豆科作物,印度在干豆产量方面位居世界第一,绿豆产量位居世界第四(联合国粮食及农业组织统计数据库,2017年)。然而,这种产量受到绿豆黄花叶印度病毒(MYMIV)感染的严重影响。因此,鉴定耐MYMIV的品种非常重要。MYMIV感染会导致活性氧的产生,植物细胞已在不同水平进化出复杂的防御机制以克服这种损害。我们的研究首次聚焦于九个品种在感染MYMIV后形态和生化水平的变化,以及MYMIV基因的相对定量。MYMIV感染后,‘Anupam’品种的生长最高且四种抗氧化剂的积累量最高,这表明在所有九个品种中,‘Anupam’品种受MYMIV感染的影响较小。相对定量研究也与这些结果高度相关。此外,在早期感染期间,‘Anupam’品种经模拟处理和MYMIV处理的幼苗中光合色素含量保持一致水平。综合所有结果,我们得出结论,‘Anupam’品种是耐MYMIV的品种。

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