Division of Plant Biology, Bose Institute, Kolkata, 700054, India.
Ramakrishna Mission Vivekananda Centenary College, Rahara, Kolkata, 7000118, India.
Sci Rep. 2019 Jun 20;9(1):8858. doi: 10.1038/s41598-019-45383-w.
Mungbean Yellow Mosaic India Virus (MYMIV)-infection creates major hindrance in V. mungo cultivation and poses significant threat to other grain legume production. Symptoms associated include severe patho-physiological alterations characterized by chlorotic foliar lesion accompanied by reduced growth. However, dissection of the host's defense machinery remains a tough challenge due to limited of host's genomic resources. A comparative RNA-Seq transcriptomes of resistant (VM84) and susceptible (T9) plants was carried out to identify genes potentially involved in V. mungo resistance against MYMIV. Distinct gene expression landscapes were observed in VM84 and T9 with 2158 and 1679 differentially expressed genes (DEGs), respectively. Transcriptomic responses in VM84 reflect a prompt and intense immune reaction demonstrating an efficient pathogen surveillance leading to activation of basal and induced immune responses. Functional analysis of the altered DEGs identified multiple regulatory pathways to be activated or repressed over time. Up-regulation of DEGs including NB-LRR, WRKY33, ankyrin, argonaute and NAC transcription factor revealed an insight on their potential roles in MYMIV-resistance; and qPCR validation shows a propensity of their accumulation in VM84. Analyses of the current RNA-Seq dataset contribute immensely to decipher molecular responses that underlie MYMIV-resistance and will aid in the improvement strategy of V. mungo and other legumes through comparative functional genomics.
绿豆黄花叶病毒（MYMIV）的感染给绿豆的种植带来了严重的阻碍，对其他豆类作物的生产也构成了重大威胁。受感染的症状包括严重的病理生理变化，表现为叶片出现黄斑，并伴随着生长受阻。然而，由于宿主基因组资源有限，解析宿主的防御机制仍然是一个艰巨的挑战。本研究对抗性（VM84）和敏感（T9）品种的绿豆进行了比较 RNA-Seq 转录组分析，以鉴定可能参与绿豆抗 MYMIV 的基因。在 VM84 和 T9 中观察到了明显不同的基因表达图谱，分别有 2158 个和 1679 个差异表达基因（DEGs）。VM84 的转录组反应反映了一种快速而强烈的免疫反应，表明其对病原体的监测效率很高，从而激活了基础免疫和诱导免疫反应。对变化的 DEGs 的功能分析表明，多个调控途径被激活或随时间抑制。DEGs 的上调，包括 NB-LRR、WRKY33、锚蛋白、argonaute 和 NAC 转录因子，揭示了它们在 MYMIV 抗性中的潜在作用；qPCR 验证表明它们在 VM84 中的积累倾向。对当前 RNA-Seq 数据集的分析极大地有助于解析 MYMIV 抗性的分子反应机制，并将通过比较功能基因组学为绿豆和其他豆类的改良策略提供帮助。
