Seo Min-Soo, Kim Young-In, Kang Kyung-Ku, Oh Se-Kyung, Sung Soo-Eun, Jung Young-Suk, Cho Joon Yong, Song HyunKeun, Hwang Dae Youn, Park Sang-Joon, Kim Kil Soo
1Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Korea.
KPC Coporation, Gwangju, 12773 Korea.
Lab Anim Res. 2019 Jul 26;35:11. doi: 10.1186/s42826-019-0010-4. eCollection 2019.
Botulinum-toxin A (BoNT/A) is a widely used not only for cosmetics but also for various experimental purposes including muscle-related research. In this study, we applied BoNT/A to mouse muscle of three different sources to compare and evaluate the biological and pathological response. The three different mouse sources consist of Korl:ICR (Korea FDA source), A:ICR (USA source) and B:ICR (Japan source) which were purchased from each different vendors. To compare the responses of ICR mice with BoNT/A muscle injection, we examined the body weight, hematological and serum biochemistry analysis. Also, we evaluated the muscle change by histopathological analysis and gene expression patterns of muscle-related target by qPCR. The body weight gain was decreased in the BoNT/A-treated group compared with the control group. In clinical pathologic analysis and gene expression patterns, the data showed that the responses in the BoNT/A-treated group were similar compared with the control group. Decreased muscle fiber was observed in BoNT/A-treated group compared with control group, while Korl:ICR showed a little low response with the other mouse sources. In conclusion, our results suggest that three different sources ICR mice (Korl:ICR, A:ICR and B:ICR) have a similar biological and pathological responses in BoNT/A muscle injection.
肉毒杆菌毒素A（BoNT/A）不仅广泛应用于化妆品领域，还用于包括肌肉相关研究在内的各种实验目的。在本研究中，我们将BoNT/A应用于三种不同来源的小鼠肌肉，以比较和评估其生物学和病理学反应。这三种不同的小鼠来源包括从不同供应商处购买的Korl:ICR（韩国食品药品监督管理局来源）、A:ICR（美国来源）和B:ICR（日本来源）。为了比较BoNT/A肌肉注射对ICR小鼠的反应，我们检测了体重、血液学和血清生化分析。此外，我们通过组织病理学分析评估了肌肉变化，并通过qPCR检测了肌肉相关靶点的基因表达模式。与对照组相比，BoNT/A治疗组的体重增加减少。在临床病理分析和基因表达模式方面，数据显示BoNT/A治疗组与对照组的反应相似。与对照组相比，BoNT/A治疗组观察到肌纤维减少，而Korl:ICR与其他小鼠来源相比反应略低。总之，我们的结果表明，三种不同来源的ICR小鼠（Korl:ICR、A:ICR和B:ICR）在BoNT/A肌肉注射中具有相似的生物学和病理学反应。
