Valet J P, Lafleur L, Marceau N
Ann Immunol (Paris). 1977 Jan-Mar;128(1-2):201-3.
Albumin-secretins hepatocytes were detected as plaque-forming cells when incubated in a complete culture medium containing sheep eythrocytes coated with purified anti-albumin, plus anti-albumin serum and complement added at appropriate dilutions. Plaque-formation is observed after incubation at 37 degrees C for 1 h 1/2 between two properly spaced microscope slides sealed with paraffin. Plaque enumeration revealed that 17-18% of normal adult rat lover cells secreted albumin. The assay which is reproductible and highly sensitive is also done in a single step.
当在含有用纯化抗白蛋白包被的绵羊红细胞的完全培养基中培养，再加入适当稀释的抗白蛋白血清和补体时，白蛋白分泌型肝细胞被检测为蚀斑形成细胞。在37℃孵育1个半小时后，在两片用石蜡密封且间距合适的显微镜载玻片之间观察到蚀斑形成。蚀斑计数显示，17 - 18%的正常成年大鼠肝细胞分泌白蛋白。该检测方法具有可重复性且高度灵敏，并且一步即可完成。
