抗凝剂诱导血小板囊泡形成的透射电子显微镜研究。
A transmission electron microscopy study of anticoagulant-induced platelet vesiculation.
作者信息
Almhanawi Bahaa Hadi, Khalid Bahariah, Ibrahim Tengku Azmi, Tohit Eusni Rahayu Mohd
机构信息
Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia.
Department of Medicine, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia.
出版信息
Porto Biomed J. 2017 Jan-Feb;2(1):23-27. doi: 10.1016/j.pbj.2016.11.002. Epub 2016 Dec 27.
HIGHLIGHTS
EDTA induces platelet microparticles (PMPs) vesiculation.Sodium citrated tubes are better than EDTA tubes in PMPs analysis.Sodium citrate has a minor impact on platelet structure.
ABSTRACT
Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to compare the direct effects of ethylenediaminetetraacetic acid (EDTA) and sodium citrate anticoagulants on platelet structure and PMP vesiculation using transmission electron microscopy to visualize the morphologic changes in platelets. Micrographs revealed that platelets in the EDTA-anticoagulated tube manifested with significant morphologic changes and induced PMP vesiculation. On the other hand, the sodium citrate-anticoagulated tube showed a normal platelet structure and minor modifications in some cases, with poor indication of PMP vesiculation. In conclusion, EDTA induced platelet activation and PMP vesiculation and represents a major source of artifacts during the pre-analysis steps of PMP vesiculation.
要点
乙二胺四乙酸（EDTA）可诱导血小板微粒（PMPs）形成囊泡。在PMPs分析中，枸橼酸钠管优于EDTA管。枸橼酸钠对血小板结构影响较小。
摘要
血小板微粒（PMPs）是活化血小板在各种刺激下释放的小膜碎片。PMPs作为多种疾病和病症的生物标志物，是用于预后、诊断和治疗目的的有用工具。我们研究的目的是使用透射电子显微镜观察血小板的形态变化，比较乙二胺四乙酸（EDTA）和枸橼酸钠抗凝剂对血小板结构和PMPs囊泡形成的直接影响。显微照片显示，EDTA抗凝管中的血小板表现出明显的形态变化并诱导PMPs囊泡形成。另一方面，枸橼酸钠抗凝管显示血小板结构正常，在某些情况下有轻微改变，PMPs囊泡形成的迹象不明显。总之，EDTA诱导血小板活化和PMPs囊泡形成，是PMPs囊泡形成分析前步骤中主要的假象来源。
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