Codon usage bias affects α-amylase mRNA level by altering RNA stability and cytosine methylation patterns in .

Codon usage bias exists in almost every organism and is reported to regulate protein translation efficiency and folding. Besides translation, the preliminary role of codon usage bias on gene transcription has also been revealed in some eukaryotes such as . In this study, we took as an example the α-amylase-coding gene () and examined the role of codon usage bias in regulating gene expression in the typical prokaryote . We confirmed the higher translation efficiency on codon-optimized RNAs and found that the RNA level itself was also affected by codon optimization. The decreased RNA level was caused at least in part by altered mRNA stability at the post-transcriptional level. Codon optimization also altered the number of cytosine methylation sites. Examination on knockouts suggested that cytosine methylation may be a minor mechanism adopted by codon bias to regulate gene RNA levels. More studies are required to verify the global effect of codon usage and to reveal its detailed mechanism on transcription.