Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
Department of Orthopedics, Yixing Second People's Hospital, Yixing 214221, China.
Life Sci. 2020 Jul 1;252:117642. doi: 10.1016/j.lfs.2020.117642. Epub 2020 Apr 4.
To determine whether ginsenoside Rg1 is involved in scratch wound healing through altered expression of related molecules in astrocytes and improved functional recovery after spinal cord injury (SCI).
Astrocytes were isolated from rats, followed by Rg1 treatment. The wound healing test was performed to observe the scratch wound healing in different groups. The expression of nerve growth factor (NGF), glial cell line-derived neurotrophic factor (GDNF), basic fibroblast growth factor (bFGF), and components of the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway were detected by western blot. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the altered expression of laminin (LN) and fibronectin (FN). A revised Allen's method for the SCI model was performed, followed by Rg1 treatment. Then, functional scoring was conducted to evaluate the functional recovery. Hematoxylin-eosin (HE) staining showed changes in the void area. Finally, western blot assessed the expression of glial fibrillary acidic protein (GFAP) and chondroitin sulfate proteoglycans (CSPGs).
Rg1 mediated scratch wound healing through inducing an increased release of LN, FN, NGF, GDNF, and bFGF in vitro. Additionally, Rg1 activated the PI3K/Akt signaling pathway and promoted the functional recovery of hindlimb movement in rats. Furthermore, Rg1 significantly reduced the void area and downregulated the expression of GFAP and CSPGs.
Rg1 not only enhanced the scratch wound repair in vitro through the release of astroglial neurotrophic factors, adhesion factors, and inhibitory factors, but it also improved the functional recovery in vivo following SCI.
通过改变星形胶质细胞中相关分子的表达，确定人参皂苷 Rg1 是否参与划痕伤口愈合，并改善脊髓损伤（SCI）后的功能恢复。
分离大鼠星形胶质细胞，并用 Rg1 处理。进行划痕愈合试验以观察不同组的划痕伤口愈合。通过 Western blot 检测神经生长因子（NGF）、胶质细胞源性神经营养因子（GDNF）、碱性成纤维细胞生长因子（bFGF）和磷酸肌醇 3-激酶（PI3K）/蛋白激酶 B（Akt）信号通路的组成部分的表达。逆转录-聚合酶链反应（RT-PCR）用于测量层粘连蛋白（LN）和纤维连接蛋白（FN）的改变表达。采用改良的 Allen 法建立 SCI 模型，并用 Rg1 处理。然后进行功能评分以评估功能恢复。苏木精-伊红（HE）染色显示空洞区域的变化。最后，Western blot 评估神经胶质纤维酸性蛋白（GFAP）和软骨素硫酸蛋白聚糖（CSPGs）的表达。
Rg1 通过体外诱导 LN、FN、NGF、GDNF 和 bFGF 的释放来介导划痕伤口愈合。此外，Rg1 激活了 PI3K/Akt 信号通路，并促进了大鼠后肢运动功能的恢复。此外，Rg1 显著减少了空洞区域，并下调了 GFAP 和 CSPGs 的表达。
Rg1 不仅通过释放星形胶质细胞神经营养因子、粘附因子和抑制因子增强了体外划痕伤口修复，而且还改善了 SCI 后体内的功能恢复。
