Departamento de Caracterización, Calidad y Seguridad, Instituto de Ciencia y Tecnología de Alimentos y Nutrición, ICTAN-CSIC, Ciudad Universitaria, E-28040, Madrid, Spain.
Departamento de Caracterización, Calidad y Seguridad, Instituto de Ciencia y Tecnología de Alimentos y Nutrición, ICTAN-CSIC, Ciudad Universitaria, E-28040, Madrid, Spain.
Plant Physiol Biochem. 2020 Jun;151:334-341. doi: 10.1016/j.plaphy.2020.03.049. Epub 2020 Mar 30.
Phenolic compounds, such as phytoalexin resveratrol, can be induced in grapes in response to biotic and abiotic stresses and have been related in many healthy effects. Stilbene synthases (STSs) are the key enzyme responsible for resveratrol biosynthesis. They have been already isolated and characterized from several plant species, however, VviSTS is a multigene family and little is known about their modulation in response to the application of gaseous treatments that maintain table grapes quality during postharvest. In this work, we have analyzed the effect of a 3-day CO treatment on the modulation of 4 STSs (VviSTS6, VviSTS7, VviSTS16 and VviSTS46) and on the accumulation of different stilbene compounds (resveratrol, resveratrol-glucoside, trans-piceatannol, z-miyabenol and pallidol) during the postharvest storage at 0 °C of white (Superior Seedless, Dominga), red (Red Globe) and black (Autumn Royal) table grapes. Results indicated that the accumulation of the stilbene compounds by the application of CO and low temperature storage were cultivar dependent. In white Dominga fruit, accumulation of stilbene compounds increased in CO-treated samples what seems to be modulated by VviSTS6, VviSTS7 and VviSTS46. However, in Red Globe the accumulation of compounds was mainly due to the cold storage in air and seems to be also mediated by the induction of the same VviSTSs. By contrast, in Superior Seedless and Autumn Royal table grapes the modulation of VviSTSs genes and the stilbene accumulation was independent of the atmosphere storage. Further studies would be needed to elucidate the possible role of transcription factors involved on VviSTSs modulation.
酚类化合物，如植物抗毒素白藜芦醇，可以在葡萄中被诱导产生，以应对生物和非生物胁迫，并与许多健康益处有关。芪合酶（STSs）是负责白藜芦醇生物合成的关键酶。它们已经从几种植物物种中分离和鉴定出来，但是 VviSTS 是一个多基因家族，对于它们在应对气体处理后的调节知之甚少，这些气体处理可以在葡萄采后保持其品质。在这项工作中，我们分析了 3 天 CO 处理对 4 种 STS（VviSTS6、VviSTS7、VviSTS16 和 VviSTS46）的调节作用，以及对不同芪类化合物（白藜芦醇、白藜芦醇葡萄糖苷、反式-比卡醇、z-米亚贝醇和苍白醇）积累的影响，在 0°C 下对白葡萄（Superior Seedless、Dominga）、红葡萄（Red Globe）和黑葡萄（Autumn Royal）进行采后贮藏。结果表明，应用 CO 和低温贮藏对芪类化合物的积累具有品种依赖性。在白葡萄 Dominga 果实中，CO 处理样品中芪类化合物的积累增加，这似乎是由 VviSTS6、VviSTS7 和 VviSTS46 调节的。然而，在红葡萄 Red Globe 中，化合物的积累主要是由于在空气中冷藏造成的，这似乎也受到相同 VviSTSs 的诱导。相比之下，在 Superior Seedless 和 Autumn Royal 葡萄中，VviSTSs 基因的调节和芪类化合物的积累与大气贮藏无关。需要进一步的研究来阐明参与 VviSTSs 调节的转录因子的可能作用。
