Sawada Toshiki, Serizawa Takeshi
Department of Organic and Polymeric Materials, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.
J Mater Chem B. 2013 Jan 14;1(2):149-152. doi: 10.1039/c2tb00066k. Epub 2012 Sep 28.
A simple dipping/sweeping-up process for the polymer substrates successfully immobilized genetically engineered filamentous viruses (M13 phages) in a highly orientated manner. The specific interaction between the peptides displayed on the phage terminus and the polymer species was essential for their orientation. The orientation manner was dependent on the sweeping-up speed and the phage concentration.
一种用于聚合物基底的简单浸渍/扫涂工艺成功地以高度定向的方式固定了基因工程丝状病毒(M13噬菌体)。噬菌体末端展示的肽与聚合物种类之间的特异性相互作用对它们的定向至关重要。定向方式取决于扫涂速度和噬菌体浓度。