Hagens S, Bläsi U
Department of Microbiology and Genetics, Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Vienna, Austria.
Lett Appl Microbiol. 2003;37(4):318-23. doi: 10.1046/j.1472-765x.2003.01400.x.
To evaluate the ability of a filamentous phage encoding lethal proteins to kill bacteria without host-cell lysis.
Bacterial survival was determined after infection of a growing Escherichia coli culture with phage M13 encoding either the restriction endonuclease BglII gene or modified phage lambda S holin genes. The genetically engineered phage exerted a high killing efficiency while leaving the cells structurally intact. When compared with a lytic phage, the release of endotoxin was minimized after infection with the genetically modified phages.
Genetically engineered phage can be used for efficient killing, concomitantly minimizing endotoxin release.
This feasibility study provides a possible strategy for the use of genetically engineered phage as bactericidal agents by optimizing the advantages and minimizing potential risks such as release of pyrogenic cell wall components.
评估编码致死蛋白的丝状噬菌体在不裂解宿主细胞的情况下杀死细菌的能力。
用编码限制性内切酶BglII基因的噬菌体M13或修饰的噬菌体λS溶菌素来感染生长中的大肠杆菌培养物后，测定细菌存活率。基因工程噬菌体具有很高的杀伤效率，同时使细胞结构保持完整。与裂解性噬菌体相比，用基因工程噬菌体感染后内毒素的释放降至最低。
基因工程噬菌体可用于高效杀菌，同时将内毒素释放降至最低。
这项可行性研究通过优化优势并将诸如热原性细胞壁成分释放等潜在风险降至最低，为使用基因工程噬菌体作为杀菌剂提供了一种可能的策略。
