基于共轭聚电解质的胰蛋白酶实时荧光开启检测法。
A real-time fluorescence turn-on assay for trypsin based on a conjugated polyelectrolyte.
作者信息
Liu Renxuan, Tan Ying, Zhang Cunlong, Wu Jiatao, Mei Lin, Jiang Yuyang, Tan Chunyan
机构信息
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.
出版信息
J Mater Chem B. 2013 Mar 14;1(10):1402-1405. doi: 10.1039/c3tb00020f. Epub 2013 Feb 1.
We report a continuous and sensitive fluorescence turn-on assay for trypsin by using an anionic conjugated polyelectrolyte (PPE-CO2H) and a cationic peptide substrate labelled with p-nitroaniline (RG-pNA). Applications of the assay in trypsin activity study and high-throughput screening of protease inhibitors were demonstrated.
我们报道了一种通过使用阴离子共轭聚电解质（PPE-CO2H）和对硝基苯胺标记的阳离子肽底物（RG-pNA）对胰蛋白酶进行连续且灵敏的荧光开启检测方法。该检测方法在胰蛋白酶活性研究和蛋白酶抑制剂的高通量筛选中的应用得到了证实。
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