Zhao Minzhi, Wang Qingsong, Lai Wenjia, Zhao Xuyang, Shen Hongyan, Nie Feilong, Zheng Yufeng, Wei Shicheng, Ji Jianguo
Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
J Mater Chem B. 2013 Apr 14;1(14):1926-1938. doi: 10.1039/c3tb00266g. Epub 2013 Feb 28.
Nanostructured titanium prepared by the equal-channel angular pressing route (ECAPed Ti) has shown great promise as an implant material over conventional pure titanium. The aim of this report is to investigate its biological properties, surface performance, and comprehensive biological effects at a molecular level when in contact with cells. Protein expression changes of human osteoblast-like MG-63 in response to polished ECAPed Ti had been profiled by employing stable isotope labelling with amino acids in cell culture (SILAC), using cpTi as control after the same polishing process. It was found that ubiquitin proteasome related processes were predominantly enriched in the over-expressed proteins. Superoxide dismutase 2 (SOD2) was apparently up-regulated on the ECAPed Ti surface, which could have contributed to the increase in SOD activity and the decrease in the reactive oxygen species (ROS) level. These expression changes have relationships with protein degradation, bone formation and resistance to oxidative injury, and they suggest that ECAPed Ti has the potential to further promote osteoblast differentiation. On the other hand, the down-regulated proteins exhibited resistance to platelet adhesion on the ECAPed Ti surface. This study reveals the differential expression of proteins in human osteoblasts induced by nanostructured titanium substrates for the first time.
通过等通道转角挤压工艺制备的纳米结构钛（ECAPed Ti）作为一种植入材料，相较于传统纯钛已展现出巨大潜力。本报告旨在研究其与细胞接触时在分子水平上的生物学特性、表面性能及综合生物学效应。采用细胞培养中氨基酸稳定同位素标记法（SILAC），以经过相同抛光处理的商业纯钛（cpTi）作为对照，分析了人成骨样细胞MG-63对抛光后的ECAPed Ti的蛋白质表达变化。结果发现，泛素蛋白酶体相关过程在过表达蛋白中显著富集。超氧化物歧化酶2（SOD2）在ECAPed Ti表面明显上调，这可能有助于超氧化物歧化酶活性的增加和活性氧（ROS）水平的降低。这些表达变化与蛋白质降解、骨形成及抗氧化损伤能力有关，表明ECAPed Ti有进一步促进成骨细胞分化的潜力。另一方面，下调的蛋白质表现出对ECAPed Ti表面血小板黏附的抗性。本研究首次揭示了纳米结构钛基底诱导人成骨细胞中蛋白质的差异表达。
