de Araújo Farias Virgínea, López-Peñalver Jesús J, Sirés-Campos Julia, López-Ramón María Victoria, Moreno-Castilla Carlos, Oliver Francisco Javier, Ruiz de Almodóvar José Mariano
Instituto de Biopatología y Medicina Regenerativa, Centro de Investigación Biomédica, Universidad de Granada, Avda. del Conocimiento 2, 18016, Granada, Spain.
J Mater Chem B. 2013 Jul 21;1(27):3359-3368. doi: 10.1039/c3tb20305k. Epub 2013 Jun 3.
We have investigated the capacity of activated carbon cloth to support the growth and differentiation of human mesenchymal umbilical-cord stromal stem cells. Our results demonstrate that this scaffold provides suitable conditions for the development of cell-derived matrix proteins and facilitates the growth of undifferentiated stem cells with the ability to induce osteogenic and chondrogenic differentiation. Immunoflourescence staining revealed extensive expression of collagen in all the samples, and collagen type II and osteopontin within the samples cultivated in specific differentiation-inducing media. Cell growth and the formation of natural collagen, calcium-magnesium carbonate and hydroxyapatite crystals, together with the self-assemblage of collagen to produce suprafibrillar arrangements of fibrils all occur simultaneously and can be studied together ex vivo under physiological conditions. Furthermore, the spontaneous differentiation of stem cells cultured on activated carbon cloth with no osteogenic supplements opens up new possibilities for bone-tumour engineering and treatment of traumatic and degenerative bone diseases.
我们研究了活性炭布支持人间充质脐带基质干细胞生长和分化的能力。我们的结果表明，这种支架为细胞衍生的基质蛋白的发育提供了合适的条件，并促进了未分化干细胞的生长，这些干细胞具有诱导成骨和成软骨分化的能力。免疫荧光染色显示所有样品中均广泛表达胶原蛋白，在特定分化诱导培养基中培养的样品中还表达II型胶原蛋白和骨桥蛋白。细胞生长以及天然胶原蛋白、碳酸钙镁和羟基磷灰石晶体的形成，以及胶原蛋白的自组装以产生超纤维状的纤维排列，这些过程同时发生，并且可以在生理条件下在体外一起进行研究。此外，在没有成骨补充剂的活性炭布上培养的干细胞的自发分化为骨肿瘤工程以及创伤性和退行性骨疾病的治疗开辟了新的可能性。
