Khan Ferdous, Valere Simon, Fuhrmann Steven, Arrighi Valeria, Bradley Mark
School of Chemistry, University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK.
J Mater Chem B. 2013 May 28;1(20):2590-2600. doi: 10.1039/c3tb00358b. Epub 2013 Apr 15.
A library of block copolymers was synthesized by varying the molecular weight of the poly(ε-caprolactone) (PCL)-diol soft segment (M = 850, 3050, 3700 or 7000), which was reacted with methylene diphenyl diisocyanate (MDI), 1,4-phenylene diisocyanate (PDI), 1,1'-methylenebis(4-isocyanatocyclohexane) (HMDI), or 2,4-toluene diisocyanate (TDI) with 1,4-butanediol (BD) or ethylene glycol (EG) added as chain extenders. Thermal and X-ray measurements indicated that the crystalline structure of the copolymers was largely dependent on the chain length of the PCL-diol, with no crystallization taking place with the smallest diol (M = 850) using MDI, TDI or HMDI. However, the copolymers produced from a PCL-diol (M = 850) and PDI and chain extenders (BD or EG) showed resolved crystalline peaks while no peaks appeared with other diisocyanates. Hydrolytic degradation studies demonstrated a faster degradation rate in the case of more amorphous copolymers than semi-crystalline copolymers. The cellular compatibility of the copolymers was evaluated by fabricating the entire library of polymers in a microarray format and in vitro cell culture, demonstrating that all the 57 copolymers supported cellular attachment and growth.
通过改变聚（ε-己内酯）（PCL）-二醇软段的分子量（M = 850、3050、3700或7000）合成了一系列嵌段共聚物，该软段与亚甲基二苯基二异氰酸酯（MDI）、1,4-亚苯基二异氰酸酯（PDI）、1,1'-亚甲基双（4-异氰酸根合环己烷）（HMDI）或2,4-甲苯二异氰酸酯（TDI）反应，并添加1,4-丁二醇（BD）或乙二醇（EG）作为扩链剂。热分析和X射线测量表明，共聚物的晶体结构在很大程度上取决于PCL-二醇的链长，使用MDI、TDI或HMDI时，最小的二醇（M = 850）不会发生结晶。然而，由PCL-二醇（M = 850）和PDI以及扩链剂（BD或EG）制备的共聚物显示出分辨出的结晶峰，而其他二异氰酸酯则没有峰出现。水解降解研究表明，与半结晶共聚物相比，更多无定形共聚物的降解速度更快。通过以微阵列形式制备整个聚合物库并进行体外细胞培养来评估共聚物的细胞相容性，结果表明所有57种共聚物都支持细胞附着和生长。
