Cao Lin, Li Xiangqing, Qin Lixia, Kang Shi-Zhao, Li Guodong
School of Chemical and Environmental Engineering, Center of Graphene Research, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, China.
J Mater Chem B. 2017 Aug 21;5(31):6300-6306. doi: 10.1039/c7tb01629h. Epub 2017 Jul 27.
A new class of cytochrome c (Cyt c) detection fluorescence sensor (GQDs-GO) based on graphene quantum dots (GQDs) supported by graphene oxide (GO) has been developed through simply mixing a certain amount of GQDs and GO. It is found that fluorescence "turn on" or "turn off" can be adjusted easily according to the concentration of Cyt c. When the concentration of Cyt c is lower than 1.9 μmol L, the fluorescence is "turn on", whereas it is "turn off" beyond 1.9 μmol L. The process is fast and can be completed within 10 s. By means of UV-vis absorption spectra, it is demonstrated that the changed fluorescence comes from the different interactions among GQDs, GO and Cyt c. Additionally, the GQDs-GO shows a high selectivity for Cyt c detection, and further exhibits favorable intracellular imaging in A549 cells. This study opens a new direction for developing a low-cost, highly efficient, GQDs-based fluorescence sensor for Cyt c detection, which make it very attractive for biological imaging applications.
通过简单混合一定量的石墨烯量子点（GQDs）和氧化石墨烯（GO），开发了一种基于氧化石墨烯负载的新型细胞色素c（Cyt c）检测荧光传感器（GQDs-GO）。研究发现，根据Cyt c的浓度可以轻松调节荧光的“开启”或“关闭”。当Cyt c浓度低于1.9 μmol/L时，荧光“开启”，而超过1.9 μmol/L时则“关闭”。该过程很快，可在10秒内完成。通过紫外可见吸收光谱表明，荧光变化源于GQDs、GO和Cyt c之间不同的相互作用。此外，GQDs-GO对Cyt c检测具有高选择性，并在A549细胞中进一步展现出良好的细胞内成像效果。本研究为开发一种低成本、高效的基于GQDs的Cyt c检测荧光传感器开辟了新方向，这使其在生物成像应用中极具吸引力。
