Wu Qiang, Xu Yueyue, Yang Kai, Cui Hailei, Chen Yanjun, Wang Menghua, Zhu Qingke, Kang Wenyi, Gao Chunsheng
College of Pharmacy, Institutes of Environment and Medicine and Chinese Material Medica, Henan University, Kaifeng, 475004, China.
J Mater Chem B. 2017 Nov 7;5(41):8219-8227. doi: 10.1039/c7tb01778b. Epub 2017 Oct 11.
In this study, several novel blend membranes based on ligand-immobilized amphiphilic carbonaceous particles (ACPs) and matrix polymer polyvinylidene fluoride (PVDF) were prepared by an immersion phase inversion method. The effects of the ACP content on filtration performance of the membrane were investigated. Endotoxin removal with blend membranes at different pH values and ionic strengths was analyzed and discussed. The results indicated that the use of ACPs not only achieved uniform and stable incorporation of water-soluble ligands into the hydrophobic polymer membrane but also improved its filtration properties, including permeability, strength, hydrophilicity and antifouling ability. The blend membranes with the optimum particle content showed good performances in filtration and were highly effective in removing endotoxins from both buffer and protein solutions under properly chosen conditions. More than 99.8% of endotoxin removal and more than 90% of BSA recovery were achieved when 1 mg L of BSA solution with 116 EU mL of the endotoxin content was treated at pH 4.7. All the above suggested that the carrier-dispersed method described herein was one of the effective methods in constructing functionalized blend membrane absorbers.
在本研究中，通过浸没相转化法制备了几种基于配体固定化两亲性碳质颗粒（ACP）和基质聚合物聚偏氟乙烯（PVDF）的新型共混膜。研究了ACP含量对膜过滤性能的影响。分析并讨论了不同pH值和离子强度下共混膜对内毒素的去除情况。结果表明，使用ACP不仅能使水溶性配体均匀、稳定地掺入疏水聚合物膜中，还能改善其过滤性能，包括渗透性、强度、亲水性和抗污染能力。具有最佳颗粒含量的共混膜在过滤方面表现良好，在适当选择的条件下，能高效地从缓冲液和蛋白质溶液中去除内毒素。当处理内毒素含量为116 EU/mL的1 mg/L BSA溶液时，在pH 4.7条件下，内毒素去除率超过99.8%，BSA回收率超过90%。上述所有结果表明，本文所述的载体分散法是构建功能化共混膜吸收剂的有效方法之一。
