Liu Peng, Zhu Bangshang, Yuan Xiaoya, Tong Gangsheng, Su Yue, Zhu Xinyuan
Instrumental Analysis Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
J Mater Chem B. 2015 Feb 21;3(7):1301-1312. doi: 10.1039/c4tb01049c. Epub 2015 Jan 7.
We investigated the effect of reaction temperature on the particle size and morphology of hydroxy zinc phosphate particles (HZnPPs). The influence of differences in shape on the physiochemical properties of HZnPPs and the possible bioapplications of these particles were also investigated. HZnPPs with both hollow and solid nanospheres and microsized rectangular sheet-like particles were successfully prepared by a wet chemical method using Zn (NO)·6HO and (NH)HPO as the reactants and ammonia to adjust the pH. The synthesis was performed at temperatures ranging from 0 to 95 °C. The particle size, morphology, crystal structure and thermal properties were analysed by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, the Brunauer-Emmett-Teller specific surface area, thermogravimetric analysis and dynamic light scatting. The chemical composition and vibrational spectra were measured using inductively coupled plasma atomic emission spectrometry, Fourier transform infrared spectrometry and Raman spectrometry. The transmission electron microscopy results showed that a hollow spherical structure with a pore size of 20-30 nm was obtained at reaction temperatures <25 °C. With increasing temperature, the nanoparticles changed from hollow to solid spheres of a similar size. Microsized rectangular sheet-like particles were formed when the reaction temperature was >60 °C. The effect of HZnPPs on cell viability in vitro was evaluated by the MTT assay against normal NIH/3T3 cells. The hollow and solid nanospheres had a lower cell cytotoxicity than the microsized sheet-like particles. Both the nanospheres and the rectangular sheet-like particles were able to adsorb heavy metal ions. The hollow nanospheres were also used as a carrier for a high drug-loading of epirubicin. These results clearly show that temperature plays an important part in regulating the nanoscale hierarchical structure of HZnPPs and their properties.
我们研究了反应温度对磷酸锌羟化物颗粒（HZnPPs）粒径和形态的影响。还研究了形状差异对HZnPPs理化性质的影响以及这些颗粒可能的生物应用。以Zn(NO₃)₂·6H₂O和(NH₄)₂HPO₄为反应物，用氨水调节pH值，通过湿化学法成功制备了具有空心和实心纳米球以及微米级矩形片状颗粒的HZnPPs。合成在0至95°C的温度范围内进行。通过X射线衍射、扫描电子显微镜、透射电子显微镜、布鲁诺尔-埃米特-泰勒比表面积、热重分析和动态光散射分析颗粒尺寸、形态、晶体结构和热性能。使用电感耦合等离子体原子发射光谱法、傅里叶变换红外光谱法和拉曼光谱法测量化学成分和振动光谱。透射电子显微镜结果表明，在反应温度<25°C时获得了孔径为20 - 30 nm的空心球形结构。随着温度升高，纳米颗粒从空心变为尺寸相似的实心球。当反应温度>60°C时形成微米级矩形片状颗粒。通过MTT法针对正常NIH/3T3细胞评估HZnPPs对体外细胞活力的影响。空心和实心纳米球的细胞毒性低于微米级片状颗粒。纳米球和矩形片状颗粒都能够吸附重金属离子。空心纳米球还被用作表柔比星高载药量的载体。这些结果清楚地表明温度在调节HZnPPs的纳米级层次结构及其性质方面起着重要作用。
