Dong Shuqi, Chen Qixian, Li Wei, Jiang Zhu, Ma Jianbiao, Gao Hui
School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, P. R. China.
J Mater Chem B. 2017 Nov 14;5(42):8322-8329. doi: 10.1039/c7tb01966a. Epub 2017 Oct 23.
Ethanolamine (EA)-functionalized poly (glycidyl methacrylate) (PGMA-EA) has been validated by our pioneering studies as a promising material for the manufacture of gene delivery systems. Herein, PGMA-EA was schemed to attach at the terminus of MOF ligands for the construction of a nanoscaled dendritic catiomer. The resulting molecular structure was arranged to possess well-defined flanking secondary amine and hydroxyl groups, which consequently exerted drastic potency in DNA condensation (approximately 100 nm) and provided appreciable colloidal stabilities in the biological milieu. Note that as compared to the commercial gold standard of branched PEI (25 kDa), the proposed catiomer exhibited markedly higher transfection efficiency because of the appreciable cationic dendritic structure and lower cytotoxicity because of rational arrangement of hydroxyl groups. Therefore, the present study encouraged the utility of an MOF-motif in engineering spatial functional and biological functional nanoscaled structures for biomedical applications.
乙醇胺（EA）功能化聚甲基丙烯酸缩水甘油酯（PGMA-EA）已被我们的开创性研究所证实，是一种用于制造基因递送系统的有前途的材料。在此，PGMA-EA被设计连接在金属有机框架（MOF）配体的末端，以构建纳米级树枝状阳离子聚合物。所得分子结构被设计为具有明确的侧翼仲胺和羟基，因此在DNA凝聚方面具有显著效力（约100纳米），并在生物环境中提供了可观的胶体稳定性。请注意，与商业化的支链聚乙烯亚胺（25 kDa）金标准相比，所提出的阳离子聚合物由于其可观的阳离子树枝状结构而表现出明显更高的转染效率，并且由于羟基的合理排列而具有更低的细胞毒性。因此，本研究鼓励在工程用于生物医学应用的空间功能和生物功能纳米级结构中使用MOF基序。
