[Value of polyacrylamide gel electrophoresis of non-concentrated cerebrospinal fluid in the diagnosis of multiple sclerosis].

Using SDS-PAGE for non-concentrated cerebrospinal fluid investigations connected with immunofixation and silver impregnation of gels the authors found oligoclonal IgG in 39 cases (95%) of clinically reliably diagnosed multiple sclerosis, and in 92.3% of probable multiple sclerosis cases. The number of IgG lines reached 13, while in the control group of neuroses it never exceeded 6-8. Comparative analysis of the IgG spectrum in cerebrospinal fluid and serum in the same multiple sclerosis cases demonstrated different IgG patterns which were only partly similar. Moreover, it was found that the electrophoretic pattern of cerebrospinal fluid proteins in each case of multiple sclerosis had an individual peculiar pattern of Ig spectrum. The use of SDS-PAGE in combination with silver impregnation made possible obtaining of qualitatively very good separation in 10-20 microliters samples of non-concentrated cerebrospinal fluid. The silver impregnation method is 50-100 times more sensitive than the traditional staining of gel with Coomasie Blue R-250. In multiple sclerosis SDS-PAGE of non-concentrated cerebrospinal fluid combined with silver impregnation of gel is a very god diagnostic method.