Grzybowski G, Wajgt A
Z I Katedry i Kliniki Neurologii Sl. AM w Katowicach.
Neurol Neurochir Pol. 1988 Jul-Aug;22(4):273-80.
Using SDS-PAGE for non-concentrated cerebrospinal fluid investigations connected with immunofixation and silver impregnation of gels the authors found oligoclonal IgG in 39 cases (95%) of clinically reliably diagnosed multiple sclerosis, and in 92.3% of probable multiple sclerosis cases. The number of IgG lines reached 13, while in the control group of neuroses it never exceeded 6-8. Comparative analysis of the IgG spectrum in cerebrospinal fluid and serum in the same multiple sclerosis cases demonstrated different IgG patterns which were only partly similar. Moreover, it was found that the electrophoretic pattern of cerebrospinal fluid proteins in each case of multiple sclerosis had an individual peculiar pattern of Ig spectrum. The use of SDS-PAGE in combination with silver impregnation made possible obtaining of qualitatively very good separation in 10-20 microliters samples of non-concentrated cerebrospinal fluid. The silver impregnation method is 50-100 times more sensitive than the traditional staining of gel with Coomasie Blue R-250. In multiple sclerosis SDS-PAGE of non-concentrated cerebrospinal fluid combined with silver impregnation of gel is a very god diagnostic method.
作者使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对未浓缩的脑脊液进行研究，并结合凝胶的免疫固定和银染法，发现在临床确诊的39例（95%）多发性硬化症患者以及92.3%的疑似多发性硬化症患者的脑脊液中存在寡克隆IgG。IgG条带数量达到13条，而在神经症对照组中，该数量从未超过6 - 8条。对同一多发性硬化症患者脑脊液和血清中的IgG谱进行比较分析，结果显示两者的IgG模式仅部分相似。此外，还发现每例多发性硬化症患者脑脊液蛋白的电泳图谱都有其独特的Ig谱模式。将SDS-PAGE与银染法结合使用，能够在10 - 20微升未浓缩脑脊液样本中实现质量上非常好的分离效果。银染法的灵敏度比传统的考马斯亮蓝R - 250凝胶染色法高50 - 100倍。在多发性硬化症的诊断中，未浓缩脑脊液的SDS-PAGE结合凝胶银染法是一种非常好的诊断方法。
