Adnan Faisal, Khan Najeeb Ullah, Iqbal Aqib, Ali Ijaz, Petruzziello Arnolfo, Sabatino Rocco, Guzzo Annunziata, Loquercio Giovanna, Botti Gerardo, Khan Sanaullah, Naeem Muhammad, Khan Muhammad Ismail
1Institute of Biotechnology and Genetic Engineering (Health Division), The University of Agriculture, Peshawar, Pakistan.
2Department of Biosciences, COMSATs University, Islamabad, Pakistan.
Infect Agent Cancer. 2020 Apr 1;15:21. doi: 10.1186/s13027-020-00285-9. eCollection 2020.
Hepatocellular carcinoma is a primary liver malignancy in which the risk of development is always multifunctional. Interleukin-6 is a proinflammatory and multifunctional cytokine, which plays an important role in the immune response, haematopoiesis and defence against viral infection. We aimed to evaluate the frequency of mutations (rs2069837 and rs17147230) associated with genetic risk of hepatocellular carcinoma in Khyber Pakthunkhwa population. A total of 72 hepatocellular carcinoma cases and 38 controls were included in this study. The genomic DNA was extracted from the peripheral blood cells and genotyping was performed using T-ARMS-PCR technique. Our results show a significant increase risk of developing hepatocellular carcinoma with the mutation within gene with heterozygous G allele (rs2069837) (OR = 10.667, 95%CI = 3.923-29.001, = < 0.0001) and heterozygous T allele (rs17147230) (OR = 75.385, 95%CI = 9.797-580.065, = < 0.0001). However, under recessive gene model the results were insignificant in case of rs2069837 (OR = 0.605, 95%CI = 0.217-1.689, = 0.337), while significant in case of rs17147230 (OR = 0.298, 95%CI = 0.121-0.734, = 0.0085). In conclusion, mutation is associated with hepatocellular carcinoma susceptibility. More related studies with other associated interleukins and their whole gene sequencing will be required.
肝细胞癌是一种原发性肝脏恶性肿瘤，其发生风险总是多因素的。白细胞介素-6是一种促炎和多功能细胞因子，在免疫反应、造血和抗病毒感染防御中起重要作用。我们旨在评估开伯尔-普赫图赫瓦省人群中与肝细胞癌遗传风险相关的突变（rs2069837和rs17147230）的频率。本研究共纳入72例肝细胞癌病例和38例对照。从外周血细胞中提取基因组DNA，并使用T-ARMS-PCR技术进行基因分型。我们的结果显示，携带杂合G等位基因（rs2069837）的基因内突变（OR = 10.667，95%CI = 3.923 - 29.001，P = < 0.0001）和杂合T等位基因（rs17147230）（OR = 75.385，95%CI = 9.797 - 580.065，P = < 0.0001）会显著增加患肝细胞癌的风险。然而，在隐性基因模型下，rs2069837的结果无统计学意义（OR = 0.605，95%CI = 0.217 - 1.689，P = 0.337），而rs17147230的结果有统计学意义（OR = 0.298，95%CI = 0.121 - 0.734，P = 0.0085）。总之，突变与肝细胞癌易感性相关。需要更多关于其他相关白细胞介素及其全基因测序的相关研究。
