Petchdee Soontaree, Laosripaiboon Wanpen, Jarussophon Nongpanga, Kumphune Sarawut
Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Bangkok, 73140, Thailand.
Department of Chemistry, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Bangkok, 73140, Thailand.
High Blood Press Cardiovasc Prev. 2020 Jun;27(3):251-258. doi: 10.1007/s40292-020-00378-x. Epub 2020 Apr 7.
Ischemic heart disease is closely associated with many risk factors. Germinated brown rice extract (GBR) has potent antioxidant activities for alleviating the factors for developing heart failure such as hypertension and diabetes mellitus.
The objective of the present study was to determine the cardio-protective effects of GBR and to elucidate the mechanisms underlying these effects in a model of simulated myocardial ischemic/ reperfusion injury (sI/R).
An in vitro study was performed on cultured rat cardiomyoblasts (H9c2) exposed to sI/R. The expression of apoptosis and signaling proteins was assessed using Western blot analyses. Eighteen New Zealand White rabbits were divided into 3 groups and the left circumflex coronary artery was ligated to induce myocardial ischemia. Heart functions were monitored by electrocardiography and echocardiography 0, 30, and 60 days after coronary artery ligation.
GBR consumption group showed significantly improved cardiac function and reduced the heart rate, along with reduced mean arterial pressure and plasma glucose level. Also, GBR showed good scavenging activity, pretreatment with GBR inhibited I/R induced apoptosis by suppressing the production of caspase 3 and p38 MAPK.
These results suggest that intake of germinated brown rice may effectively to protect cell proliferation and apoptosis and may provide important nutrients to prevent heart failure due to myocardial ischemia.
缺血性心脏病与许多危险因素密切相关。发芽糙米提取物（GBR）具有强大的抗氧化活性，可减轻诸如高血压和糖尿病等导致心力衰竭的因素。
本研究的目的是确定GBR的心脏保护作用，并阐明在模拟心肌缺血/再灌注损伤（sI/R）模型中这些作用的潜在机制。
对暴露于sI/R的培养大鼠心肌成纤维细胞（H9c2）进行体外研究。使用蛋白质免疫印迹分析评估凋亡和信号蛋白的表达。将18只新西兰白兔分为3组，结扎左旋冠状动脉以诱导心肌缺血。在冠状动脉结扎后0、30和60天通过心电图和超声心动图监测心脏功能。
GBR摄入组的心脏功能显著改善，心率降低，同时平均动脉压和血糖水平降低。此外，GBR显示出良好的清除活性，用GBR预处理通过抑制半胱天冬酶3和p38丝裂原活化蛋白激酶的产生来抑制I/R诱导的细胞凋亡。
这些结果表明，摄入发芽糙米可能有效地保护细胞增殖和凋亡，并可能提供重要的营养物质以预防心肌缺血引起的心力衰竭。
