IDiBE, Universidad Miguel Hernández, 03202, Elche, Spain.
IDiBE, Universidad Miguel Hernández, 03202, Elche, Spain.
Colloids Surf B Biointerfaces. 2020 Jul;191:110993. doi: 10.1016/j.colsurfb.2020.110993. Epub 2020 Mar 30.
The adsorption of proteins to silica surface is a common process mainly governed by the electrostatic attractive interaction between the pH-dependent negatively silica surface and the positive charges of the biomolecule. This process often reduces the conformational stability of the adsorbed protein and may reduce its biological functionality mostly due to multimolecular processes such as aggregation and fibrillation. Here we show that high-density charge cationic polyelectrolytes may successfully compete with the protein for the silica surface containing deprotonated-silanol groups. Therefore, the coating of silica surfaces with these cationic polyelectrolytes precludes the adsorption of the protein to the solid surface. Intensive water washing of the polyelectrolyte-coated silica surfaces had does not result in polyelectrolyte release (even at moderate ionic strength) maintaining the solid surface protected from protein adsorption.
硅烷表面的蛋白质吸附是一个常见的过程，主要由 pH 依赖性的硅烷表面的静电吸引相互作用和生物分子的正电荷控制。这个过程常常降低了吸附蛋白质的构象稳定性，并且可能由于多分子过程（如聚集和纤维化）降低其生物功能。在这里我们表明，高密度电荷的阳离子聚电解质可能成功地与硅烷表面的去质子化硅醇基团竞争，从而阻止蛋白质吸附到固体表面。用这些阳离子聚电解质强烈地冲洗硅烷表面，不会导致聚电解质的释放（即使在中等离子强度下），从而保持固体表面不受蛋白质吸附的影响。
