Li G W, Liu C K, Liu P, Deng T G, Li J L, Hu K J
Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University & State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Xi'an 710032, China.
Department of Oral and Maxillofacial Surgery, School of Stomatology Xi'an Medical University, Xi'an 710021, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2020 Apr 9;55(4):259-263. doi: 10.3760/cma.j.cn112144-20191129-00427.
To determine the opening and closing action of the external muscle, the projection pathway of the axon terminal of trigeminal motor nucleus (Vmo) neuron to the lateral pterygoid muscle was revealed. In this study, 10 SD rats of 8 weeks old were included. The left lateral pterygoid muscle of SD rats was surgically exposed, and the wound was closed after intramuscular injection of hydroxystilbamidine/fluorogold (FG) 3-5 μl. Seven days after the operation, the experimental animals were perfused, samples collected and sectioned for immunofluorescence staining. After FG injection into the lateral pterygoid muscle, the FG reversed in the Vmo neurons. In the Vmo neurons on the FG injection side (left side), a large number of FG reversed neurons were found in the corpus luteum and dendrites. These neurons were not only distributed in the dorsolateral part of the trigeminal motor nucleus that innervated the closed muscle, but also in the ventral medial portion of the trigeminal nucleus of the open muscle. The neuronal conduction pathway between the Vmo and the lateral pterygoid muscle innervates the lateral pterygoid muscle. The neurons are distributed both in the dorsolateral and in the nucleus of the ventral ventricle. It is concluded that the lateral pterygoid muscle involve in the jaw closing and opening movement.
为了确定翼外肌的开闭动作，揭示了三叉神经运动核（Vmo）神经元轴突终末至翼外肌的投射通路。本研究纳入10只8周龄的SD大鼠。手术暴露SD大鼠左侧翼外肌，肌肉内注射3 - 5 μl羟乙磺酰苯脒/荧光金（FG）后关闭创口。术后7天，对实验动物进行灌注，采集样本并切片进行免疫荧光染色。向翼外肌注射FG后，FG在Vmo神经元中逆行。在注射FG侧（左侧）的Vmo神经元中，在黄体和树突中发现大量FG逆行神经元。这些神经元不仅分布在支配闭口肌的三叉神经运动核的背外侧部分，也分布在支配开口肌的三叉神经核的腹内侧部分。Vmo与翼外肌之间的神经元传导通路支配翼外肌。这些神经元分布在背外侧和脑室腹侧核中。得出结论：翼外肌参与下颌的开闭运动。
