Freund R K, Martin B J, Jungschaffer D A, Ullman E A, Collins A C
Institute for Behavioral Genetics, University of Colorado, Boulder 80309.
Pharmacol Biochem Behav. 1988 Aug;30(4):1059-64. doi: 10.1016/0091-3057(88)90139-6.
Changes in plasma corticosterone (CCS) levels following intraperitoneal injections of nicotine were measured in four inbred mouse strains: DBA/2Ibg, C57BL/6Ibg, C3H/2Ibg, and A/J. In all four strains, nicotine produced a dose-dependent (0.5-2.0 mg/kg nicotine) increase in plasma CCS levels which peaked 10-30 min after injection. Saline increased plasma CCS levels in C57BL, A, and C3H, but not in DBA mice. After correcting for plasma CCS levels produced by saline injection, the nicotine-induced rise in plasma CCS was significantly lower for the C57BL strain than for the other three strains tested. These mouse strains also varied in their responses to saline injection with the rank order: C57BL greater than A = C3H greater than DBA. However, the two most divergent strains (C57BL and DBA) did not differ in the effects of a cold water stress. The response to nicotine was completely inhibited by mecamylamine in two strains tested (C3H and C57BL) whereas the response to saline injection was unaffected, suggesting that only the response to nicotine was mediated by nicotinic receptors. It is clear that elevations in plasma CCS induced either by saline injection or by nicotine are influenced by genetic factors.
在四种近交系小鼠品系（DBA/2Ibg、C57BL/6Ibg、C3H/2Ibg和A/J）中测量了腹腔注射尼古丁后血浆皮质酮（CCS）水平的变化。在所有这四种品系中，尼古丁均使血浆CCS水平呈剂量依赖性（0.5 - 2.0毫克/千克尼古丁）升高，在注射后10 - 30分钟达到峰值。生理盐水使C57BL、A和C3H品系的血浆CCS水平升高，但对DBA小鼠则不然。在对注射生理盐水所产生的血浆CCS水平进行校正后，C57BL品系中尼古丁诱导的血浆CCS升高幅度显著低于其他三种受试品系。这些小鼠品系对注射生理盐水的反应也有所不同，顺序为：C57BL大于A = C3H大于DBA。然而，两个差异最大的品系（C57BL和DBA）在冷水应激的影响方面并无差异。在两种受试品系（C3H和C57BL）中，美加明完全抑制了对尼古丁的反应，而对注射生理盐水的反应则未受影响，这表明只有对尼古丁的反应是由烟碱受体介导的。显然，注射生理盐水或尼古丁所诱导的血浆CCS升高受到遗传因素的影响。
