Whole-Genome Analysis of Two Copies of Gene Carrying Strain Acsw19 Isolated from Sichuan, China.

PURPOSE

To characterize the genetic feature of the carbapenems resistant strain Acsw19 isolated from municipal sludge. This strain was found to carry two copies of -like gene, and -like gene along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages.

METHODS

A carbapenem-resistant strain Acsw19 isolated from municipal sludge was subjected to whole-genome sequencing (WGS) via the PacBio and Illumina MiSeq platforms. Thereafter, the characteristic was analyzed by a series of bioinformatics software.

RESULTS

The results showed that the genome of Acsw19 was consisted of a 3,433,749 bp circular chromosome and 3 circular plasmids, pAcsw19-1 (11,161 bp), pAcsw19-2 (351,885 bp) and pAcsw19-3 (38,391bp), respectively. Resistome analysis showed that Acsw19 carried 12 antimicrobial resistance genes, including 6 [-like, , , () and ] in the plasmid pAcsw19-2 and 6 ( -like, , , and ) in the chromosome genome. Specifically, the -like gene shared 86.33%, 71.7% and 71.9% similarities with the and gene, and the -like gene shared 94.4%, 95.39% and 96.36% similarities with , and , at the nucleotide level, respectively. Phylogenetic analysis showed that the -like gene and -like gene had the closest evolutionary relationship with and , respectively. These results indicated that the -like and -like genes identified in the current study should be the novel variant resistance genes.

CONCLUSION

Carrying of two copies of , like, -like and along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages strain might increase the possibility of spreading of resistance genes.