Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, 610041, P. R. China.
Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, 610041, P. R. China.
J Microbiol. 2019 May;57(5):350-355. doi: 10.1007/s12275-019-8485-0. Epub 2019 Feb 26.
Two strains of the genus Acinetobacter, named WCHAc010005 and WCHAc010052, were isolated from hospital sewage at West China Hospital in Chengdu, China. The two strains were found to be resistant to carbapenems due to the presence of carbapenemase gene bla. Based on the comparative analysis of the rpoB sequence, the two strains formed a strongly supported and internally coherent cluster (intracluster identity of 98.7%), which was clearly separated from all known Acinetobacter species (≤ 83.4%). The two strains also formed a tight and distinct cluster based on the genuswide comparison of whole-cell mass fingerprints generated by MALDI-TOF mass spectrometry. In addition, the combination of their ability to assimilate malonate but not benzoate, and the inability to grow at 37°C could distinguish the two strains from all known Acinetobacter species. The two strains were subjected to whole genome sequencing using both short-read Illumina HiSeq2500 platform and the longread MinION sequencer. The average nucleotide identity and in silico DNA-DNA hybridization value between the genomes of WCHAc010005 and WCHAc010052 was 96.69% and 74.3% respectively, whereas those between the two genomes and the known Acinetobacter species were < 80% and < 30%, respectively. Therefore, the two strains represent a novel species of the genus Acinetobacter, for which the name Acinetobacter chinensis sp. nov. is proposed, and the type strain is WCHAc- 010005 (= GDMCC 1.1232 = KCTC 61813).
两株不动杆菌属菌株，命名为 WCHAc010005 和 WCHAc010052，从中国成都华西医院的医院污水中分离得到。这两株菌由于存在碳青霉烯酶基因 bla，被发现对碳青霉烯类药物具有耐药性。根据 rpoB 序列的比较分析，这两株菌形成了一个具有强烈支持力和内部一致性的聚类（聚类内同一性为 98.7%），与所有已知的不动杆菌属物种明显分离（≤83.4%）。这两株菌还根据 MALDI-TOF 质谱生成的全细胞质量指纹图谱在属水平上的比较形成了一个紧密而独特的聚类。此外，它们能够同化丙二酸盐但不能同化苯甲酸，以及不能在 37°C 下生长的能力，可以将这两株菌与所有已知的不动杆菌属物种区分开来。这两株菌使用短读 Illumina HiSeq2500 平台和长读 MinION 测序仪进行全基因组测序。WCHAc010005 和 WCHAc010052 基因组之间的平均核苷酸同一性和 DNA-DNA 杂交值的数值分别为 96.69%和 74.3%，而这两个基因组与已知的不动杆菌属物种之间的数值分别小于 80%和小于 30%。因此，这两株菌代表了不动杆菌属的一个新种，命名为 Acinetobacter chinensis sp. nov.，其模式菌株为 WCHAc-010005（=GDMCC 1.1232=KCTC 61813）。
