Bijkerk A H, Hall R J
Biotechnol Bioeng. 1977 Feb;19(2):267-96. doi: 10.1002/bit.260190209.
A two-stage deterministic model of the growth of Saccharomyces cerevisiae is presented. The cell cycle of this organism was used to suggest the basic model structure. The model represents the preparatory processes of substrate uptake and conversion separately from replication and division. The regulation of the fraction of the culture devoted to each of these broad areas of metabolism, and the overall growth rate, is related to the nature and availability of the energy substrate. The simulation of respiration and glycolysis is achieved by including two alternative energy producing pathways. The regulation of these pathways is described in terms of the postulated primary regulation of the proportion of the culture required for substrate uptake and conversion, and the overall kinetic constants for each pathway. This regulation is dictated primarily by the growth rate rather than the nature or concentration of the energy substrate. The model successfully describes both batch and continuous growth of S. cerevisiae under conditons of glucose limitation and oxygen excess. A preliminary assessment indicates that adjustment of the relevant parameters will allow the model to describe the growth of S. cerevisiae on other sugars and under oxygen limitation. Similarly the model could be expected to describe the growth characteristics of other yeast species.
本文提出了一种酿酒酵母生长的两阶段确定性模型。利用该生物体的细胞周期来构建基本模型结构。该模型将底物摄取和转化的准备过程与复制和分裂过程分开表示。致力于这些广泛代谢领域中每一个领域的培养物比例的调节以及总体生长速率，与能量底物的性质和可用性有关。通过纳入两条替代的能量产生途径来实现呼吸作用和糖酵解的模拟。这些途径的调节是根据假定的底物摄取和转化所需培养物比例的主要调节以及每条途径的总体动力学常数来描述的。这种调节主要由生长速率决定，而不是由能量底物的性质或浓度决定。该模型成功地描述了在葡萄糖限制和氧气过量条件下酿酒酵母的分批生长和连续生长。初步评估表明，调整相关参数将使该模型能够描述酿酒酵母在其他糖类上以及在氧气限制条件下的生长情况。同样，可以预期该模型能够描述其他酵母物种的生长特性。
