National Institutes of Health-NIRT-International Center for Excellence in Research, Chennai, India; National Institute for Research in Tuberculosis (NIRT), Chennai, India.
National Institutes of Health-NIRT-International Center for Excellence in Research, Chennai, India.
Tuberculosis (Edinb). 2020 May;122:101934. doi: 10.1016/j.tube.2020.101934. Epub 2020 Apr 1.
Pulmonary tuberculosis is associated with higher plasma levels of antimicrobial peptides (AMPs) and lower granulysin levels. However, the association of AMPs with tuberculous lymphadenitis (TBL) is not well studied. Hence, we measured the plasma levels of human beta defensin-2 (HBD2), granulysin, human neutrophil peptides 1-3 (HNP1-3) and cathelicidin (LL37) in TBL compared to latent tuberculosis (LTB) and healthy controls (HC) and in TBL individuals upon completion of anti-tuberculosis treatment (ATT). We examined the association of AMPs with TBL lymph node culture grade or lymph node involvement. Finally, the discriminatory potential of these proteins was assessed using receiver operating characteristic (ROC) analysis. TBL individuals display significantly diminished circulating levels of AMPs (granulysin and HNP1-3) but not HBD-2 and LL-37 in comparison to LTB and HCs. Similarly, after ATT, both HBD-2 and HNP1-3 were significantly elevated and LL-37 was significantly reduced in TBL individuals. Granulysin and HNP1-3 discriminates TBL from LTB and HC individuals upon ROC analysis. AMPs did not exhibit significant correlation either with lymph node culture grades or lymph node involvement. Overall, TBL individuals show decreased AMPs and their reversal after ATT suggesting their association with underlying immune alteration in this poorly studied form of TB disease.
肺结核与抗微生物肽（AMPs）的血浆水平升高和颗粒溶素水平降低有关。然而，AMPs 与结核性淋巴结炎（TBL）的关系尚未得到充分研究。因此，我们测量了 TBL 患者与潜伏性结核（LTB）和健康对照（HC）相比的血浆中人β防御素-2（HBD2）、颗粒溶素、人中性粒细胞肽 1-3（HNP1-3）和 cathelicidin（LL37）水平，并在 TBL 患者完成抗结核治疗（ATT）后进行了测量。我们检查了 AMPs 与 TBL 淋巴结培养分级或淋巴结受累的相关性。最后，使用接收者操作特征（ROC）分析评估了这些蛋白质的鉴别潜力。与 LTB 和 HC 相比，TBL 患者的循环 AMPs（颗粒溶素和 HNP1-3）水平明显降低，但 HBD-2 和 LL-37 水平没有降低。同样，ATT 后，TBL 患者的 HBD-2 和 HNP1-3 明显升高，LL-37 明显降低。ROC 分析显示，颗粒溶素和 HNP1-3 可将 TBL 与 LTB 和 HC 个体区分开来。AMP 与淋巴结培养分级或淋巴结受累均无显著相关性。总的来说，TBL 患者表现出 AMPs 减少，ATT 后逆转，表明它们与这种研究较少的结核病形式中潜在的免疫改变有关。
