Angeles Ros M, Ojeda J L, García-Porrero J A
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Cantabria, Santander, Spain.
Acta Anat (Basel). 1988;133(4):303-8. doi: 10.1159/000146658.
The patterns of silver affinity and following treatment with guanidine were studied in the basement membrane produced by pareital podocytes induced by corticoids in newborn rabbits. The goal of this study was to analyze the role of the different cell types of the renal corpuscle in the determination of the histochemical characteristics of the glomerular basement membrane (GBM). Jones' method shows that while the GBM exhibited silver affinity only after periodic-acid oxidation, the basement membrane of parietal podocytes exhibits the same histochemical characteristics as the normal parietal basement membrane, appearing deep black both after periodic-acid or permanganate oxidation, and after elastase or lysozyme digestions. Since the treatment with guanidine shows that the basement membrane of the parietal podocytes lacks the endothelial component typical of the GBM, it may be suggested that the special resistance to silver impregnation exhibited by the basement membrane after permanganate oxidation or after different enzymatic digestions is due to its endothelial component.
研究了新生兔皮质激素诱导的壁层足细胞产生的基底膜中的银亲和模式以及随后用胍处理的情况。本研究的目的是分析肾小体不同细胞类型在肾小球基底膜（GBM）组织化学特征确定中的作用。琼斯方法表明，虽然GBM仅在高碘酸氧化后表现出银亲和性，但壁层足细胞的基底膜与正常壁层基底膜具有相同的组织化学特征，在高碘酸或高锰酸钾氧化后以及弹性蛋白酶或溶菌酶消化后均呈现深黑色。由于用胍处理表明壁层足细胞的基底膜缺乏GBM典型的内皮成分，因此可以认为，高锰酸钾氧化后或不同酶消化后基底膜对银浸染表现出的特殊抗性是由于其内皮成分。
