作为纳米传感器用于次氯酸根检测的聚集诱导发光的执行及在活细胞和斑马鱼中的生物成像应用。
Execution of aggregation-induced emission as nano-sensors for hypochlorite detection and application for bioimaging in living cells and zebrafish.
机构信息
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China; Luoyang Key Laboratory of Organic Functional Molecules, College of Food and Drug, Luoyang Normal University, Luoyang, 471934, China.
School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
出版信息
Talanta. 2020 Jul 1;214:120842. doi: 10.1016/j.talanta.2020.120842. Epub 2020 Feb 19.
Hypochlorite (ClO) could be used as a diagnostic marker for inflammation and related diseases. Although there have been many reports on probes for ClO imaging, there was still a lack of specificity and anti-interference ability. Herein, carbazole (NEC) and tetraphenylethylene (TPE) equipped with thiobarbituric acid (TBA), NEC-TBA and TPE-TBA, were synthesized and used as a fluorescence biosensor for monitoring ClO with aggregation-induced emission (AIE) effect. we identified that TPE-TBA, with formed nanoparticles in the mean grain size at 76 nm (5 μM), was a superior probe to target ClO over other analytes with fluorescence "turn off" strategy. Subsequently, to explore the bioimaging application, TPE-TBA was able to sense exogenous ClO in living HeLa cells through fluorescence imaging. In zebrafish model, TPE-TBA effectively captured exogenous ClO in the entire organization of zebrafish. Overall, these AIE-based probes merit further development as organism targeting ClO sensors.
次氯酸盐 (ClO) 可作为炎症和相关疾病的诊断标志物。尽管已经有很多关于 ClO 成像探针的报道，但仍缺乏特异性和抗干扰能力。在此，我们合成了带有硫代巴比妥酸 (TBA) 的咔唑 (NEC) 和四苯基乙烯 (TPE)，即 NEC-TBA 和 TPE-TBA，并将其用作具有聚集诱导发射 (AIE) 效应的荧光生物传感器，用于监测 ClO。我们发现，TPE-TBA 在 5 μM 时形成平均粒径为 76nm 的纳米颗粒，是一种优于其他分析物的探针，具有荧光“关闭”策略，可靶向 ClO。随后，为了探索生物成像应用，TPE-TBA 能够通过荧光成像在活 HeLa 细胞中检测外源性 ClO。在斑马鱼模型中，TPE-TBA 有效地捕获了斑马鱼整个组织中的外源性 ClO。总的来说，这些基于 AIE 的探针值得进一步开发，作为靶向 ClO 的生物传感器。
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