All-Russian Research Institute for Optical and Physical Measurements, 46 Ozernaya st., 119361, Moscow, Russia.
Moscow Institute of Physics and Technology, 9 Institutskii per., 141700, Dolgoprudny, Moscow Region, Russia.
Anal Bioanal Chem. 2020 May;412(14):3423-3431. doi: 10.1007/s00216-020-02605-9. Epub 2020 Apr 12.
A new approach to direct quantitative detection of small molecules (haptens) by dynamic light scattering biosensing is presented. The proposed technique implements a homogeneous competitive immunoassay and is based on optical detection of specific inhibition of nanoparticle aggregation induced by the analyte in a sample. The technique performance was tested both in buffer and milk for detection of chloramphenicol - antibiotic relevant to food safety diagnostics. Good specificity, sensitivity (LOD in milk is 2.4 ng/ml), precision (4.0 ± 1.2%), ruggedness (8.3%), and 96% recovery in conjunction with a record wide dynamic range (3 orders of magnitude) of the nanosensing technique were demonstrated. Such characteristics complemented by the assay simplicity (no washing step) and a short assay time make the approach attractive for application as an analytical platform for point-of-care and field-oriented diagnostics. Graphical abstract.
一种新的方法，通过动态光散射生物传感直接定量检测小分子（半抗原）。该方法采用均相竞争免疫测定法，基于光学检测分析物在样品中特异性抑制纳米颗粒聚集。该技术在缓冲液和牛奶中均进行了测试，用于检测与食品安全诊断相关的氯霉素抗生素。该纳米传感技术具有良好的特异性、灵敏度（牛奶中的检测限为 2.4ng/ml）、精密度（4.0±1.2%）、稳健性（8.3%）和 96%的回收率，并且具有创纪录的宽动态范围（3 个数量级）。该方法具有简单的测定步骤（无需洗涤步骤）和较短的测定时间，与分析平台的吸引力互补，可用于即时诊断和现场诊断。
