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热处理羊奶对酪蛋白胶束大小、酸凝胶流变学和质构特性以及凝固型酸奶的影响。

Influence of heat treatment of goat milk on casein micelle size, rheological and textural properties of acid gels and set type yoghurts.

机构信息

Department of Animal Source Food Technology, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia.

Department of Chemistry and Biochemistry, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia.

出版信息

J Texture Stud. 2020 Aug;51(4):680-687. doi: 10.1111/jtxs.12524. Epub 2020 Apr 27.

Abstract

Acid gels and yoghurts were made from goat milk that was heated at 72°C/30 s, 85°C/5 min, and 95°C/5 min, followed by acidification with starter culture at 43C until pH 4.6. The rheological and textural properties of acid gels and yoghurts were analyzed using dynamic low amplitude oscillatory rheology and back extrusion texture analysis, respectively. The effect of goat milk heat treatment on the mean casein micelle diameter and protein profile was also determined by dynamic light scattering and SDS PAGE electrophoresis, respectively. The shortest gelation and fermentation time was recorded for yoghurt prepared from milk heated at 85°C/5 min. Also, the pH of gelation, the storage moduli (G') and yield stress were higher for this yoghurt, compared with the other two. Textural properties of goat milk yoghurts such as firmness and consistency were strongly affected by milk heat treatment, and the highest values were recorded for yoghurt produced from milk preheated at 85°C/5 min, as well. The largest casein micelles were measured after 85°C/5 min treatment and their size decreased at higher temperature, despite higher denaturation of whey proteins at the most intense heat regime, indicating the structure changes that influence on the acid gelation.

摘要

酸凝胶和酸奶是用加热到 72°C/30 秒、85°C/5 分钟和 95°C/5 分钟的山羊奶制成的,然后用发酵剂在 43°C 酸化,直到 pH 值达到 4.6。使用动态低幅度振荡流变学和反向挤出质地分析分别分析酸凝胶和酸奶的流变学和质地特性。分别通过动态光散射和 SDS-PAGE 电泳确定山羊奶热处理对平均酪蛋白胶束直径和蛋白质图谱的影响。从加热至 85°C/5 分钟的牛奶制备的酸奶记录了最短的凝胶化和发酵时间。此外,与其他两种相比,该酸奶的凝胶化 pH 值、储存模量(G')和屈服应力更高。山羊奶酸奶的质地特性,如硬度和稠度,受牛奶热处理的强烈影响,在 85°C/5 分钟预热的牛奶中生产的酸奶具有最高的值。在 85°C/5 分钟处理后测量到最大的酪蛋白胶束,并且其尺寸在更高的温度下减小,尽管在最强烈的热条件下乳清蛋白的变性更高,表明影响酸凝胶化的结构变化。

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