Matsuo S, Takano Y, Wakisaka S, Ichikawa H, Nishikawa S, Akai M
First Department of Oral Anatomy, Osaka University Faculty of Dentistry, Japan.
Anat Rec. 1988 Dec;222(4):317-22. doi: 10.1002/ar.1092220403.
The budding of small vesicles from the rough-surfaced endoplasmic reticulum (rER) was examined in the secretory ameloblast of rat molar tooth germs by ordinary fixation or prolonged osmium fixation. The budding of small vesicles from the rER was observed not only at the special region (transitional region) of the rER system, which abutted on the cis-face of the Golgi apparatus, but also at other regions of the rER in the secretory ameloblast. Small vesicles (presumed to be transitional vesicles) were adjacent to the rER, which also showed budding of vesicles. After prolonged osmium treatment, osmium deposits appeared in small vesicles, as well as in the cisternae of the cis saccule of the Golgi apparatus. Small vesicles containing osmium deposits were located at various regions of the cell, including the cis-face of the Golgi apparatus. These findings indicate that the budding of small vesicles from the rER is not restricted to the transitional region of the rER system of the secretory ameloblast, but is found at various regions of the cell. This indicates that newly synthesized proteins may be transferred from the rER cisternae to the transitional vesicles not only in the transitional region of the rER system adjacent to the Golgi apparatus, but also in other regions of the secretory ameloblast.
通过常规固定或延长锇固定，研究了大鼠磨牙牙胚分泌性成釉细胞中糙面内质网（rER）上小囊泡的出芽情况。不仅在rER系统与高尔基体顺面相邻的特殊区域（过渡区域）观察到了rER上小囊泡的出芽，在分泌性成釉细胞的rER其他区域也观察到了。小囊泡（推测为过渡囊泡）与rER相邻，rER也显示出囊泡出芽。延长锇处理后，在小囊泡以及高尔基体顺面扁平囊泡的潴泡中均出现了锇沉淀。含有锇沉淀的小囊泡位于细胞的各个区域，包括高尔基体的顺面。这些发现表明，rER上小囊泡的出芽并不局限于分泌性成釉细胞rER系统的过渡区域，而是在细胞的各个区域都能发现。这表明新合成的蛋白质不仅可以在与高尔基体相邻的rER系统过渡区域从rER潴泡转移到过渡囊泡中，还可以在分泌性成釉细胞的其他区域进行转移。
