The Institute of Medical Science and Department of Microbiology, China Medical University Hospital, Taichung, Taiwan.
Infection Control Office, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.
Microb Drug Resist. 2020 Sep;26(9):1050-1057. doi: 10.1089/mdr.2019.0410. Epub 2020 Apr 13.
Carbapenemase-producing combined porin loss is one of the primary mechanisms for carbapenem resistance. Although mutations in and genes have often been identified in carbapenem-resistant , reports on the porin protein gene disruption by insertion sequence (IS) elements are varied. The porin protein gene disruption by IS elements and OmpK36 production loss in six -carrying isolates were detected in this study. IS, IS and IS insertions were noted in the 3, 2, and 1 isolates, respectively. The six isolates showed five different pulsed-field gel electrophoresis patterns and belonged to four multilocus sequence typing types, ST4, ST11, ST15, and ST39. This study increases our understanding of the genetic background of KPC-2 carbapenemases in porin-defective clinical isolates and the contribution of OmpK36 production loss to carbapenem resistance.
产碳青霉烯酶的孔蛋白缺失是碳青霉烯类耐药的主要机制之一。虽然在碳青霉烯类耐药菌中经常发现 和 基因突变，但关于插入序列（IS）元件引起孔蛋白基因中断的报道却各不相同。本研究检测到 6 株携带 基因的菌株中存在 IS 元件引起的 孔蛋白基因中断和 OmpK36 产生缺失。在 3、2 和 1 株菌中分别观察到 IS、IS 和 IS 的插入。这 6 株分离株表现出 5 种不同的脉冲场凝胶电泳图谱，属于 4 种多位点序列分型类型，即 ST4、ST11、ST15 和 ST39。本研究加深了我们对产 KPC-2 碳青霉烯酶的孔蛋白缺陷临床分离株的遗传背景以及 OmpK36 产生缺失对碳青霉烯类耐药性的贡献的理解。
