China Pharmaceutical University, Nanjing, 210009, China.
Research Institute of Nanjing Chemical Industry Group, Nanjing, 210048, China.
J Hazard Mater. 2020 Jul 15;394:122531. doi: 10.1016/j.jhazmat.2020.122531. Epub 2020 Mar 25.
This work provided a comprehensive perspective to investigate the performance of NaHCO-driving effect and mechanism including the antibiotic removal, degradation pathway and metabolites analysis, and the algal physiological response during the removal process. Cefuroxime sodium was selected as the target antibiotic. Our results showed that NaHCO did not facilitate self-decomposition of the target antibiotic, while drove the improvement on the removal capacity of every algal cell, which then attributed to the total removal efficiency. After 24 h, there was an improvement on the removal rate of the target antibiotic (from 10.21% to 92.89%) when NaHCO was added. The degradation pathway of the target antibiotic was confirmed by the formation of three main products (M1, M2 and M3), and the degradation process, that from M1 to M2 and M2 to M3, was accelerated by the existence of NaHCO. Besides, a 4-stage model illustrated the relationship between NaHCO and antibiotic removal process. Moreover, algal culture that supplemented with NaHCO demonstrated a better growth capacity. A large increase in the content of chlorophyll a and a moderate increase in the activity of two carbon metabolic enzymes (RuBisCO and CA) might be viewed as a positive response of the algae during the NaHCO-driving process.
这项工作提供了一个全面的视角来研究 NaHCO 驱动效应及其机制，包括抗生素去除、降解途径和代谢物分析，以及在去除过程中藻类的生理反应。头孢呋辛钠被选为目标抗生素。我们的结果表明，NaHCO 并没有促进目标抗生素的自分解，而是提高了每个藻类细胞的去除能力，从而提高了总去除效率。在 24 小时后，添加 NaHCO 后，目标抗生素的去除率提高了（从 10.21%提高到 92.89%）。通过形成三种主要产物（M1、M2 和 M3），确定了目标抗生素的降解途径，并且 NaHCO 的存在加速了从 M1 到 M2 和 M2 到 M3 的降解过程。此外，还提出了一个四阶段模型来描述 NaHCO 和抗生素去除过程之间的关系。此外，补充 NaHCO 的藻类培养显示出更好的生长能力。叶绿素 a 含量的大量增加和两种碳代谢酶（RuBisCO 和 CA）的适度增加，可能被视为藻类在 NaHCO 驱动过程中的一种积极反应。
