Satué Katiuska, Fazio Esterina, Medica Pietro
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, CEU-Cardenal Herrera University, 46115 Valencia, Spain.
Department of Veterinary Sciences, Veterinary Physiology Unit, Polo Universitario Annunziata, Messina University, 98168 Messina, Italy.
Animals (Basel). 2020 Apr 9;10(4):646. doi: 10.3390/ani10040646.
The hypothesis of this study was to investigate if the presence of corpus luteum (CL) in one ovary could modify the hormonal content of follicular fluid (FF) in the follicles. Sixty ovaries were taken after the slaughter of 30 clinically healthy mares. In relation to the sizes, the follicles were classified into three different categories, as small (20-30 mm), medium (31-40 mm) and large (≥41 mm). Blood samples were collected from the jugular vein of mares before their slaughter, and then the FF samplings were extracted from each single follicle. The ovaries that were collected were classified into two groups, according to the presence (CL-bearing) or absence (non-CL-bearing) of CL. The serum and FF samples were analysed for progesterone (P), oestradiol-17β (E), testosterone (T), androstenedione (A) and dehydroepiandrosterone (DHEA). Intrafollicular P concentrations in large follicles of CL-bearing groups were lower than for non-CL-bearing ones. Intrafollicular E concentrations increased with the increase of the follicle diameter in both groups, CL-bearing and non-CL-bearing. However, in the FF with a large and medium follicle size, E concentrations were significantly higher in non-CL-bearing groups than in CL-bearing groups. T and A significantly increased in the large and medium follicle sizes when compared to the small follicle sizes in both groups, but higher concentrations in the non-CL-bearing group were obtained. Intrafollicular DHEA significantly decreased with the increase of the follicular diameter in both groups. Steroid hormones in FF dynamically changed, according to the presence or not of CL in the ovary. This study brings new knowledge on the role of the CL in the follicular hormonal composition in mares.
本研究的假设是调查一侧卵巢中黄体（CL）的存在是否会改变卵泡中卵泡液（FF）的激素含量。在屠宰30匹临床健康母马后采集了60个卵巢。根据大小，卵泡被分为三类，即小卵泡（20 - 30毫米）、中卵泡（31 - 40毫米）和大卵泡（≥41毫米）。在母马屠宰前从其颈静脉采集血样，然后从每个单个卵泡中提取FF样本。根据CL的存在（含CL）或不存在（不含CL），将采集的卵巢分为两组。对血清和FF样本进行孕酮（P）、雌二醇 - 17β（E）、睾酮（T）、雄烯二酮（A）和脱氢表雄酮（DHEA）分析。含CL组大卵泡内的卵泡内P浓度低于不含CL组。两组（含CL组和不含CL组）卵泡内E浓度均随卵泡直径增加而升高。然而，在中、大卵泡大小的FF中，不含CL组的E浓度显著高于含CL组。与两组中的小卵泡大小相比，中、大卵泡大小的T和A显著增加，但不含CL组获得的浓度更高。两组卵泡内DHEA均随卵泡直径增加而显著降低。FF中的类固醇激素根据卵巢中CL的存在与否而动态变化。本研究为CL在母马卵泡激素组成中的作用带来了新知识。
