Snf5 和 Swi3 亚基复合物的形成是酵母中 SWI/SNF 复合物功能所必需的。
Snf5 and Swi3 subcomplex formation is required for SWI/SNF complex function in yeast.
机构信息
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin, 300071, China.
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin, 300071, China.
出版信息
Biochem Biophys Res Commun. 2020 Jun 11;526(4):934-940. doi: 10.1016/j.bbrc.2020.03.169. Epub 2020 Apr 10.
The SWI/SNF chromatin remodeling complex, which alters nucleosome positions by either evicting histones or sliding nucleosomes on DNA, is highly conserved from yeast to humans, and 20% of all human cancers have mutations in various subunits of the SWI/SNF complex. Here, we reported the crystal structure of the yeast Snf5-Swi3 subcomplex at a resolution of 2.65 Å. Our results showed that the Snf5-Swi3 subcomplex assembles into a heterotrimer with one Snf5 molecule bound to two distinct Swi3 molecules. In addition, we demonstrated that Snf5-Swi3 subcomplex formation is required for SWI/SNF function in yeast. These findings shed light on the important role of the Snf5-Swi3 subcomplex in the assembly and functional integrity of the SWI/SNF complex.
SWI/SNF 染色质重塑复合物通过驱逐组蛋白或在 DNA 上滑动核小体来改变核小体位置，从酵母到人高度保守，并且 20%的人类癌症在 SWI/SNF 复合物的各种亚基中具有突变。在这里，我们报道了酵母 Snf5-Swi3 亚基复合物的晶体结构，分辨率为 2.65Å。我们的结果表明，Snf5-Swi3 亚基复合物组装成一个异三聚体，一个 Snf5 分子结合到两个不同的 Swi3 分子上。此外，我们证明了 Snf5-Swi3 亚基复合物的形成对于酵母中 SWI/SNF 功能是必需的。这些发现揭示了 Snf5-Swi3 亚基复合物在 SWI/SNF 复合物的组装和功能完整性中的重要作用。
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