Tshishonga Khuthadzo, Serepa-Dlamini Mahloro Hope
Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Johannesburg, South Africa.
Evol Bioinform Online. 2020 Apr 6;16:1176934320913257. doi: 10.1177/1176934320913257. eCollection 2020.
strain MHSD1 is a bacterial endophyte isolated from sterilized leaves of , a medicinal plant capable of growing in arid environments. Here, we report the draft genome sequence and annotation of this bacterial endophyte. The draft genome sequence of strain MHSD1 has 4 450 468 bp with a G + C content of 65.30%. The National Center for Biotechnology Information Prokaryotic Genome Annotation Pipeline identified a total of 4004 protein-coding genes, 56 genes coding for RNAs, and 82 pseudogenes. Biosynthesis pathways for various phytohormones such as auxin, salicylic acid, ethylene, cytokinin, jasmonic acid, abscisic acid, and gibberellins were identified. Putative genes involved in various characteristics of bacterial endophyte lifestyle such as transport, motility, adhesion, membrane proteins, secretion and delivery systems, plant cell wall modification, and detoxification were identified. Phylogenomic analysis showed strain MHSD1 to be a subspecies of Sphe3.
菌株MHSD1是从一种能够在干旱环境中生长的药用植物的无菌叶片中分离出的一种细菌内生菌。在此,我们报告该细菌内生菌的基因组草图序列及注释。菌株MHSD1的基因组草图序列有4450468 bp,G+C含量为65.30%。美国国立生物技术信息中心原核生物基因组注释管道共鉴定出4004个蛋白质编码基因、56个编码RNA的基因和82个假基因。鉴定出了生长素、水杨酸、乙烯、细胞分裂素、茉莉酸、脱落酸和赤霉素等各种植物激素的生物合成途径。鉴定出了与细菌内生菌生活方式的各种特征相关的推定基因,如转运、运动性、粘附、膜蛋白、分泌和递送系统、植物细胞壁修饰和解毒。系统基因组分析表明菌株MHSD1是Sphe3的一个亚种。