Nishinaka Takashi, Yamazaki Yui, Niwa Atsuko, Wake Hidenori, Mori Shuji, Yoshino Tadashi, Nishibori Masahiro, Takahashi Hideo
Department of Pharmacology, Faculty of Medicine, Kindai University, Osaka, Japan.
Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
Biomarkers. 2020 May;25(3):305-313. doi: 10.1080/1354750X.2020.1750703. Epub 2020 Apr 14.
Cerebral small vessel disease (CSVD) is associated with future stroke. Although pathological alteration in small vessels of patients with CSVD can be detected by neuroimaging, diagnosis of CSVD is delayed because it is an asymptomatic disease. The stroke-prone spontaneously hypertensive rat (SHRSP) show similar pathological features to human CSVD and develop stroke-related symptoms with advancing age. We investigated the time course of haematological parameters in Wistar rats and SHRSP. Blood cells were analysed using an automated haematological analyser. SHRSP develop stroke-related symptoms including onset of neurological symptoms, decreased body weight and blood brain barrier leakage between 12 and 14 weeks of age. Lymphocyte counts were gradually decreased at 3 weeks before development of stoke-related symptoms and then were further decreased after the development of stroke-related symptoms. The both mean platelet volume and large platelet ratio gradually increased at 3 weeks before the development of stoke-related symptoms. However, although SHRSP showed more microcytic red cells than Wistar rats, the trajectories of change in erythrocyte-related parameters were similar between Wistar rats and SHRSP. Our pilot study suggests that alterations of lymphocyte count and platelet volume predictive indicators for asymptomatic CSVD and symptomatic stroke in SHRSP.
脑小血管病（CSVD）与未来发生的中风有关。虽然通过神经影像学可以检测到CSVD患者小血管的病理改变，但由于CSVD是一种无症状疾病，其诊断往往被延迟。易中风自发性高血压大鼠（SHRSP）表现出与人类CSVD相似的病理特征，并随着年龄的增长出现与中风相关的症状。我们研究了Wistar大鼠和SHRSP血液学参数的时间进程。使用自动血液分析仪分析血细胞。SHRSP在12至14周龄时出现与中风相关的症状，包括神经症状发作、体重减轻和血脑屏障渗漏。在出现中风相关症状前3周，淋巴细胞计数逐渐下降，在出现中风相关症状后进一步下降。平均血小板体积和大血小板比率在出现中风相关症状前3周均逐渐升高。然而，尽管SHRSP的小红细胞比Wistar大鼠多，但Wistar大鼠和SHRSP红细胞相关参数的变化轨迹相似。我们的初步研究表明，淋巴细胞计数和血小板体积的改变可作为SHRSP无症状CSVD和症状性中风的预测指标。
