Department of Materials Science and Engineering Southern University of Science and Technology, Shenzhen, 518055, China.
State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China.
Chem Commun (Camb). 2020 May 19;56(40):5405-5408. doi: 10.1039/d0cc00579g.
The first NIR fluorescent mitochondria-targeting K+ sensor, denoted as TAC-Rh, was developed. The produced sensor consists of a rhodamine analog as the fluorophore and triazacryptand (TAC) as the K+ recognition unit. Compared to the K+ sensors reported previously, TAC-Rh exhibits two unique optical properties: the largest Stokes shifts (120 nm) and the longest emission peak wavelength (720 nm). With the assistance of this novel sensor, real-time changes of K+ concentrations in mitochondria during apoptosis were monitored for the first time. Moreover, it was also the first time that the relationship between mitochondrial K+ flux and apoptosis was investigated in real time using fluorescence imaging.
首次研制出一种近红外荧光靶向线粒体的钾离子传感器，命名为 TAC-Rh。该传感器由罗丹明类似物作为荧光团和三氮唑笼（TAC）作为钾离子识别单元组成。与之前报道的钾离子传感器相比，TAC-Rh 具有两个独特的光学特性：最大的斯托克斯位移（120nm）和最长的发射峰波长（720nm）。借助这种新型传感器，首次实时监测到细胞凋亡过程中线粒体中钾离子浓度的变化。此外，这也是首次利用荧光成像实时研究线粒体钾离子流与细胞凋亡之间的关系。
