Mulé S J, Jukofsky D, Kogan M, De Pace A, Verebey K
Clin Chem. 1977 May;23(5):796-801.
The 125I-radioimmunoassay (RIA) for benzylecgonine (cocaine metabolite) in urine was evaluated by comparison with gas-liquid chromatography (GLC) and thin-layer chromatography (TLC) and the Enzyme-Multiplied Immunoassay Technique (EMIT). By RIA, a statistically significant concentration, 2 microng/liter, was observed for urinary benzoylecgonine. The coefficient of variation for the RIA was 2.58+/-0.38% inter-assay and 2.20+/-0.14% intraassay. There was cross-reactivity with cocaine (more reactive than benzoylecgonine) and other members of the tropane family of alkaloids. There was agreement between results by RIA and GLC in 95.5% of the samples, between RIA and TLC in 87.0%, and between RIA and EMIT in 84.5%. The percentage of true false-positives was 3.5% for the RIA in comparison to GLC, 8.8% in comparison to TLC, and 9.1% in comparison to EMIT. True false-negatives were insignificant (0 to 1.0%). GLC and RIA results correlated highly (phi=0.908). GLC, therefore, was the best comparison method for this evaluation study. RIA for benzoylecgonine is sensitive, reproducible, and reliable for the detection of cocaine in urine.
通过与气液色谱法（GLC）、薄层色谱法（TLC）以及酶免疫倍增技术（EMIT）进行比较，对尿液中苯甲酰爱康宁（可卡因代谢物）的125I放射免疫测定法（RIA）进行了评估。通过RIA法，观察到尿液中苯甲酰爱康宁的浓度具有统计学意义，为2微克/升。RIA法的批间变异系数为2.58±0.38%，批内变异系数为2.20±0.14%。它与可卡因（比苯甲酰爱康宁更具反应性）以及托烷类生物碱家族的其他成员存在交叉反应。RIA法与GLC法的结果在95.5%的样本中一致，与TLC法在87.0%的样本中一致，与EMIT法在84.5%的样本中一致。与GLC法相比，RIA法的真阳性率为3.5%，与TLC法相比为8.8%，与EMIT法相比为9.1%。真阴性率不显著（0至1.0%）。GLC法和RIA法的结果高度相关（phi = 0.908）。因此，GLC法是该评估研究的最佳比较方法。用于检测尿液中可卡因的苯甲酰爱康宁RIA法灵敏、可重复且可靠。
