Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100085, China.
Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Beijing Key Laboratory of Industrial Wastewater Treatment and Reuse, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Environ Pollut. 2020 Aug;263(Pt A):114525. doi: 10.1016/j.envpol.2020.114525. Epub 2020 Apr 7.
Organophosphate flame retardants (OPFRs) are widespread in the aquatic environment, but the effects of these chemicals on reproductive toxicity are far from clear. In this study, sperm quality in adult male Chinese rare minnows after exposure to tris-(2-butoxyethyl) phosphate (TBOEP), tris-(1,3-dichloro-2-propyl) phosphate (TDCIPP), and triphenyl phosphate (TPHP) was investigated. No obvious change in sperm concentration and vitality was observed after treatments, whereas significant changes in sperm velocity and morphology were found following all treatments (P < 0.05). Moreover, OPFR exposure significantly increased the apoptosis ratios in testis cells. Analysis of the transcriptomic data revealed that Na/K ATPase (NKA) related genes were significantly downregulated, and the NKA enzyme activities after all treatments were significantly inhibited (P < 0.05). However, no obvious change in hormone levels in the groups exposed to TBOEP and TDCIPP was observed. These findings indicate that the OPFR-induced reduction of sperm quality might be due to the effects of OPFRs on NKA enzyme instead of changes in hormone levels.
有机磷阻燃剂（OPFRs）广泛存在于水生态环境中，但这些化学物质对生殖毒性的影响尚不清楚。本研究探讨了三（2-丁氧基乙基）磷酸酯（TBOEP）、三（1,3-二氯-2-丙基）磷酸酯（TDCIPP）和磷酸三苯酯（TPHP）暴露对成年雄性中国稀有鲫精子质量的影响。处理后，精子浓度和活力未见明显变化，但所有处理组的精子速度和形态均发生显著变化（P<0.05）。此外，OPFR 暴露显著增加了睾丸细胞的凋亡比例。转录组数据分析显示，Na/K ATPase（NKA）相关基因显著下调，所有处理组的 NKA 酶活性均显著受到抑制（P<0.05）。然而，暴露于 TBOEP 和 TDCIPP 的组的激素水平没有明显变化。这些发现表明，OPFR 引起的精子质量下降可能是由于 OPFR 对 NKA 酶的影响，而不是激素水平的变化。
